aPKC-mediated displacement and actomyosin-mediated retention polarize Miranda in Drosophila neuroblasts

Matthew Robert Hannaford, Anne Ramat, Nicolas Loyer, Jens Januschke (Lead / Corresponding author)

Research output: Contribution to journalArticle

12 Citations (Scopus)
161 Downloads (Pure)

Abstract

Cell fate assignment in the nervous system of vertebrates and invertebrates often hinges on the unequal distribution of molecules during progenitor cell division. We address asymmetric fate determinant localization in the developing Drosophila nervous system, specifically the control of the polarized distribution of the cell fate adapter protein Miranda. We reveal a step-wise polarization of Miranda in larval neuroblasts and find that Miranda’s dynamics and cortical association are differently regulated between interphase and mitosis. In interphase Miranda binds to the plasma membrane. Then, before nuclear envelope breakdown, Miranda is phosphorylated by aPKC and displaced into the cytoplasm. This clearance is necessary for the subsequent establishment of asymmetric Miranda localization. After nuclear envelope breakdown, actomyosin activity is required to maintain Miranda asymmetry. Therefore, phosphorylation by aPKC and differential binding to the actomyosin network are required at distinct phases of the cell cycle to polarize fate determinant localization in neuroblasts.
Original languageEnglish
Article numbere29939
Number of pages22
JournaleLife
Volume7
DOIs
Publication statusPublished - 24 Jan 2018

Keywords

  • Actomyosin/metabolism
  • Animals
  • Cell Cycle Proteins/metabolism
  • Drosophila Proteins/metabolism
  • Drosophila/growth & development
  • Larva/growth & development
  • Neurons/physiology
  • Phosphorylation
  • Protein Binding
  • Protein Kinase C/metabolism
  • Protein Processing, Post-Translational
  • Stem Cells/physiology

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  • Student Theses

    Establishing and Maintaining Cortical Asymmetry in Drosophila Neural Stem Cells

    Author: Hannaford, M., 2017

    Supervisor: Januschke, J. (Supervisor)

    Student thesis: Doctoral ThesisDoctor of Philosophy

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    Januschke, Jens

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