Application of a Sensitive and Reproducible Label-Free Proteomic Approach to Explore the Proteome of Individual Meiotic-Phase Barley Anthers

Dominika Lewandowska, Runxuan Zhang, Isabelle Colas, Nicola Uzrek, Robbie Waugh (Lead / Corresponding author)

Research output: Contribution to journalArticle

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Abstract

Meiosis is a highly dynamic and precisely regulated process of cell division, leading to the production of haploid gametes from one diploid parental cell. In the crop plant barley (Hordeum vulgare), male meiosis occurs in anthers, in specialized cells called meiocytes. Barley meiotic tissue is scarce and not easily accessible, making meiosis study a challenging task. We describe here a new micro-proteomics workflow that allows sensitive and reproducible genome-wide label-free proteomic analysis of individual staged barley anthers. This micro-proteomic approach detects more than 4,000 proteins from such small amounts of material as two individual anthers, covering a dynamic range of protein relative abundance levels across five orders of magnitude. We applied our micro-proteomics workflow to investigate the proteome of the developing barley anther containing pollen mother cells in the early stages of meiosis and we successfully identified 57 known and putative meiosis-related proteins. Meiotic proteins identified in our study were found to be key players of many steps and processes in early prophase such as: chromosome condensation, synapsis, DNA double-strand breaks or crossover formation. Considering the small amount of starting material, this work demonstrates an important technological advance in plant proteomics and can be applied for proteomic examination of many size-limited plant specimens. Moreover, it is the first insight into the proteome of individual barley anther at early meiosis. The proteomic data have been deposited to the ProteomeXchange with the accession number PXD010887.

Original languageEnglish
Article number393
Pages (from-to)1-15
Number of pages15
JournalFrontiers in Plant Science
Volume10
DOIs
Publication statusPublished - 2 Apr 2019

Fingerprint

proteome
proteomics
anthers
meiosis
barley
proteins
prophase
cells
haploidy
Hordeum vulgare
cell division
germ cells
diploidy
pollen
chromosomes
genome
DNA
crops

Keywords

  • Anthers
  • Label-free micro-proteomics
  • Mass spectrometry LC-MS/MS
  • Meiocytes
  • Meiosis
  • Plants

Cite this

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title = "Application of a Sensitive and Reproducible Label-Free Proteomic Approach to Explore the Proteome of Individual Meiotic-Phase Barley Anthers",
abstract = "Meiosis is a highly dynamic and precisely regulated process of cell division, leading to the production of haploid gametes from one diploid parental cell. In the crop plant barley (Hordeum vulgare), male meiosis occurs in anthers, in specialized cells called meiocytes. Barley meiotic tissue is scarce and not easily accessible, making meiosis study a challenging task. We describe here a new micro-proteomics workflow that allows sensitive and reproducible genome-wide label-free proteomic analysis of individual staged barley anthers. This micro-proteomic approach detects more than 4,000 proteins from such small amounts of material as two individual anthers, covering a dynamic range of protein relative abundance levels across five orders of magnitude. We applied our micro-proteomics workflow to investigate the proteome of the developing barley anther containing pollen mother cells in the early stages of meiosis and we successfully identified 57 known and putative meiosis-related proteins. Meiotic proteins identified in our study were found to be key players of many steps and processes in early prophase such as: chromosome condensation, synapsis, DNA double-strand breaks or crossover formation. Considering the small amount of starting material, this work demonstrates an important technological advance in plant proteomics and can be applied for proteomic examination of many size-limited plant specimens. Moreover, it is the first insight into the proteome of individual barley anther at early meiosis. The proteomic data have been deposited to the ProteomeXchange with the accession number PXD010887.",
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author = "Dominika Lewandowska and Runxuan Zhang and Isabelle Colas and Nicola Uzrek and Robbie Waugh",
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Application of a Sensitive and Reproducible Label-Free Proteomic Approach to Explore the Proteome of Individual Meiotic-Phase Barley Anthers. / Lewandowska, Dominika; Zhang, Runxuan; Colas, Isabelle; Uzrek, Nicola; Waugh, Robbie (Lead / Corresponding author).

In: Frontiers in Plant Science, Vol. 10, 393, 02.04.2019, p. 1-15.

Research output: Contribution to journalArticle

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AU - Lewandowska, Dominika

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