Application of new optical coherence elastography to monitor the mineralization processing in bone tissue engineering constructs

Guangying Guan, Shaozhen Song, Zhihong Huang, Ying Yang (Lead / Corresponding author)

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    Generation of functional tissue in vitro through tissue engineering technique is a promising direction to repair and replace malfunctioned organ and tissue in the modern medicine for various diseases which could not been treated well by conventional therapy. Similar to the embryo development, the generation of tissue in vitro is a highly dynamic processing. Obtaining the feedback of the processing real time is highly demanded. In this study, a new methodology has been explored aiming to monitor the morphological and mechanical property alteration of bone tissue engineering constructs simultaneously. Optical coherence elastography (OCE) equipped with a LDS V201 permanent magnet shaker and a modulated acoustic radiation force (ARF) to provide a vibration signal, has been used for the real time and non-destructive monitoring. A phantom construct system has been used to optimize the measurement conditions in which agar hydrogel with concentration from 0, 0.75 to 2% with/without hydroxyappatite particles have been injected to 3D porous poly (lactic acid) scaffolds to simulate the collagenous extracellular matrix (ECM) and mineralized ECM. The structural and elastography images of the constructs have clearly demonstrated the linear relation with the increased mechanical property versus the increase of agar concentration within the pores of the scaffolds. The MG63 bone cells seeded in the scaffolds and cultured for 4 weeks have been monitored by the established protocol exhibiting the increased mechanical strength in the pore wall where the ECM or mineralized ECM was assumed to be formed in comparison to empty pores. This study confirms that OCE-ARF could become a valuable tool in regenerative medicine to assess the biological events during in vitro culture and conditioning.

    Original languageEnglish
    Title of host publicationDynamics and Fluctuations in Biomedical Photonics XII
    EditorsValery V. Tuchin, Kirill V. Larin, Martin J. Leahy, Ruikang K. Wang
    PublisherSPIE-International Society for Optical Engineering
    Number of pages8
    Volume9322
    ISBN (Print)9781628414127
    DOIs
    Publication statusPublished - 3 Apr 2015
    EventSPIE Photonics West 2015: Dynamics and Fluctuations in Biomedical Photonics XII - Moscone Center, San Francisco, United States
    Duration: 7 Feb 20158 Feb 2015
    http://spie.org/conferences-and-exhibitions/past-conferences-and-exhibitions/photonics-west-2015 (Link to Conference details)

    Conference

    ConferenceSPIE Photonics West 2015
    CountryUnited States
    CitySan Francisco
    Period7/02/158/02/15
    Internet address

    Keywords

    • ECM
    • Mineralization
    • Optical coherence elastography
    • Tissue engineering bone
    • Young's modulus

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