Projects per year
Abstract
Drosophila is an important model system to study a vast range of biological questions. Various organs and tissues from different developmental stages of the fly such as imaginal discs, the larval brain or egg chambers of adult females or the adult intestine can be extracted and kept in culture for imaging with time-lapse microscopy, providing valuable insights into cell and developmental biology. Here, we describe in detail our current protocol for the dissection of Drosophila larval brains, and then present our current approach for immobilizing and orienting larval brains and other tissues on a glass coverslip using Fibrin clots. This immobilization method only requires the addition of Fibrinogen and Thrombin to the culture medium. It is suitable for high-resolution time lapse imaging on inverted microscopes of multiple samples in the same culture dish, minimizes the lateral drifting frequently caused by movements of the microscope stage in multi-point visiting microscopy and allows for the addition and removal of reagents during the course of imaging. We also present custom-made macros that we routinely use to correct for drifting and to extract and process specific quantitative information from time-lapse analysis.
Original language | English |
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Article number | e61954 |
Number of pages | 21 |
Journal | JoVE: Journal of Visualized Experiments |
Volume | 166 |
DOIs | |
Publication status | Published - 22 Dec 2020 |
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Dive into the research topics of 'Applications of Immobilization of Drosophila Tissues with Fibrin Clots for Live Imaging'. Together they form a unique fingerprint.Projects
- 2 Finished
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Development of Live Tissue Imaging Capabilities Using Multiphoton Confocal Microscopy
Muller, A. (Investigator), Nathke, I. (Investigator), Storey, K. (Investigator) & Weijer, K. (Investigator)
30/06/14 → 29/06/19
Project: Research
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Recycling Polarity - Mechanisms Controlling Stem Cell Polarity in Consecutive Divisions in the Developing Drosophila Central Nervous System (Sir Henry Dale Fellowship)
Januschke, J. (Investigator) & Storey, K. (Investigator)
1/02/13 → 30/11/21
Project: Research
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Dissecting cell polarity with chemical genetics
Januschke, J. (Speaker)
10 Aug 2021Activity: Talk or presentation types › Keynote
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Dissecting cel polarity with chemical genetics
Januschke, J. (Speaker)
4 May 2021Activity: Talk or presentation types › Keynote
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Asymmetric division of Drosophila neural stem cells
Januschke, J. (Speaker)
22 Sept 2020Activity: Talk or presentation types › Invited talk