Arabidopsis RNA immunoprecipitation

Lionel C. Terzi, Gordon G. Simpson (Lead / Corresponding author)

    Research output: Contribution to journalArticle

    35 Citations (Scopus)

    Abstract

    P>RNA-binding proteins are key regulators of plant gene expression. Consistent with this, the Arabidopsis genome encodes many RNA-binding proteins that are genetically required for normal development and for responding to environmental changes. However, the direct RNA targets and RNA processing events that these RNA-binding proteins control are poorly understood. In order to facilitate the functional characterization of RNA-binding proteins, we have applied the RNA immunoprecipitation assay to Arabidopsis. Working with the U2B '-U2 snRNA interaction as a model experimental system, we show that treatment of intact plants with formaldehyde allows immunocapture of U2 snRNA using antibodies that recognize U2B ' fused to the generic GFP tag. When coupled with recent developments in whole-genome tiling arrays and high-throughput next-generation sequencing, this combination of procedures and technology has the potential to allow systematic functional analysis of plant RNA-binding proteins.

    Original languageEnglish
    Pages (from-to)163-168
    Number of pages6
    JournalPlant Journal
    Volume59
    Issue number1
    DOIs
    Publication statusPublished - Jul 2009

    Keywords

    • Arabidopsis
    • post-transcriptional
    • RNA-binding protein
    • RNA immunoprecipitation
    • RNA
    • BINDING PROTEINS
    • MESSENGER-RNA
    • FLOWERING TIME
    • THALIANA
    • METHYLATION
    • SPECIFICITY
    • ENCODES
    • BRAIN

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