Array-based sequencing of filaggrin gene for comprehensive detection of disease-associated variants

X. F. Colin C. Wong, Simon L. I. J. Denil, Jia Nee Foo, Huijia Chen, Angeline Su Ling Tay, Rebecca L. Haines, Mark B. Y. Tang, W. H. Irwin McLean, Aileen Sandilands, Frances J. D. Smith, E. Birgitte Lane, Jianjun Liu, John E. A. Common

Research output: Contribution to journalLetter

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Abstract

Comprehensive sequencing of FLG is a challenging endeavour. We have developed a method using array-based amplicon PCR and NGS for a robust and cost efficient method to analyze this major atopic dermatitis risk factor.

Original languageEnglish
Pages (from-to)814-816
Number of pages3
JournalJournal of Allergy and Clinical Immunology
Volume141
Issue number2
Early online date19 Oct 2017
DOIs
Publication statusPublished - Feb 2018

Fingerprint

Atopic Dermatitis
Genes
Costs and Cost Analysis
Polymerase Chain Reaction
filaggrin

Keywords

  • Atopic dermatitis
  • Filaggrin
  • Mutations
  • Microfluidics
  • NGS
  • Skin

Cite this

Wong, X. F. C. C., Denil, S. L. I. J., Foo, J. N., Chen, H., Ling Tay, A. S., Haines, R. L., ... Common, J. E. A. (2018). Array-based sequencing of filaggrin gene for comprehensive detection of disease-associated variants. Journal of Allergy and Clinical Immunology, 141(2), 814-816. https://doi.org/10.1016/j.jaci.2017.10.001
Wong, X. F. Colin C. ; Denil, Simon L. I. J. ; Foo, Jia Nee ; Chen, Huijia ; Ling Tay, Angeline Su ; Haines, Rebecca L. ; Tang, Mark B. Y. ; McLean, W. H. Irwin ; Sandilands, Aileen ; Smith, Frances J. D. ; Lane, E. Birgitte ; Liu, Jianjun ; Common, John E. A. / Array-based sequencing of filaggrin gene for comprehensive detection of disease-associated variants. In: Journal of Allergy and Clinical Immunology. 2018 ; Vol. 141, No. 2. pp. 814-816.
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title = "Array-based sequencing of filaggrin gene for comprehensive detection of disease-associated variants",
abstract = "Comprehensive sequencing of FLG is a challenging endeavour. We have developed a method using array-based amplicon PCR and NGS for a robust and cost efficient method to analyze this major atopic dermatitis risk factor.",
keywords = "Atopic dermatitis, Filaggrin, Mutations, Microfluidics, NGS, Skin",
author = "Wong, {X. F. Colin C.} and Denil, {Simon L. I. J.} and Foo, {Jia Nee} and Huijia Chen and {Ling Tay}, {Angeline Su} and Haines, {Rebecca L.} and Tang, {Mark B. Y.} and McLean, {W. H. Irwin} and Aileen Sandilands and Smith, {Frances J. D.} and Lane, {E. Birgitte} and Jianjun Liu and Common, {John E. A.}",
note = "This work was funded by A*STAR SPF grants for basic and translational skin research (IAF SPF 2013/004; IAF SPF 2013/005) to J.E.A.C., E.B.L., J.N.F., S.L.I.J.D. and J.L., and A*STAR SPF genetic orphan diseases (IAF SPF 2012/005) to X.F.C.C.W. A.S and F.D.J.S. were supported by a Wellcome Trust Strategic Award (098439) to W.H.I.M",
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language = "English",
volume = "141",
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Wong, XFCC, Denil, SLIJ, Foo, JN, Chen, H, Ling Tay, AS, Haines, RL, Tang, MBY, McLean, WHI, Sandilands, A, Smith, FJD, Lane, EB, Liu, J & Common, JEA 2018, 'Array-based sequencing of filaggrin gene for comprehensive detection of disease-associated variants', Journal of Allergy and Clinical Immunology, vol. 141, no. 2, pp. 814-816. https://doi.org/10.1016/j.jaci.2017.10.001

Array-based sequencing of filaggrin gene for comprehensive detection of disease-associated variants. / Wong, X. F. Colin C.; Denil, Simon L. I. J.; Foo, Jia Nee; Chen, Huijia; Ling Tay, Angeline Su; Haines, Rebecca L.; Tang, Mark B. Y.; McLean, W. H. Irwin; Sandilands, Aileen; Smith, Frances J. D.; Lane, E. Birgitte; Liu, Jianjun; Common, John E. A.

In: Journal of Allergy and Clinical Immunology, Vol. 141, No. 2, 02.2018, p. 814-816.

Research output: Contribution to journalLetter

TY - JOUR

T1 - Array-based sequencing of filaggrin gene for comprehensive detection of disease-associated variants

AU - Wong, X. F. Colin C.

AU - Denil, Simon L. I. J.

AU - Foo, Jia Nee

AU - Chen, Huijia

AU - Ling Tay, Angeline Su

AU - Haines, Rebecca L.

AU - Tang, Mark B. Y.

AU - McLean, W. H. Irwin

AU - Sandilands, Aileen

AU - Smith, Frances J. D.

AU - Lane, E. Birgitte

AU - Liu, Jianjun

AU - Common, John E. A.

N1 - This work was funded by A*STAR SPF grants for basic and translational skin research (IAF SPF 2013/004; IAF SPF 2013/005) to J.E.A.C., E.B.L., J.N.F., S.L.I.J.D. and J.L., and A*STAR SPF genetic orphan diseases (IAF SPF 2012/005) to X.F.C.C.W. A.S and F.D.J.S. were supported by a Wellcome Trust Strategic Award (098439) to W.H.I.M

PY - 2018/2

Y1 - 2018/2

N2 - Comprehensive sequencing of FLG is a challenging endeavour. We have developed a method using array-based amplicon PCR and NGS for a robust and cost efficient method to analyze this major atopic dermatitis risk factor.

AB - Comprehensive sequencing of FLG is a challenging endeavour. We have developed a method using array-based amplicon PCR and NGS for a robust and cost efficient method to analyze this major atopic dermatitis risk factor.

KW - Atopic dermatitis

KW - Filaggrin

KW - Mutations

KW - Microfluidics

KW - NGS

KW - Skin

U2 - 10.1016/j.jaci.2017.10.001

DO - 10.1016/j.jaci.2017.10.001

M3 - Letter

VL - 141

SP - 814

EP - 816

JO - Journal of Allergy and Clinical Immunology

JF - Journal of Allergy and Clinical Immunology

SN - 0091-6749

IS - 2

ER -