Array-based sequencing of filaggrin gene for comprehensive detection of disease-associated variants

X. F. Colin C. Wong; Simon L. I. J. Denil; Jia Nee Foo; Huijia Chen; Angeline Su Ling Tay; Rebecca L. Haines; Mark B. Y. Tang; W. H. Irwin McLean; Aileen Sandilands; Frances J. D. Smith; E. Birgitte Lane; Jianjun Liu; John E. A. Common

doi:10.1016/j.jaci.2017.10.001

Array-based sequencing of filaggrin gene for comprehensive detection of disease-associated variants

X. F. Colin C. Wong, Simon L. I. J. Denil, Jia Nee Foo, Huijia Chen, Angeline Su Ling Tay, Rebecca L. Haines, Mark B. Y. Tang, W. H. Irwin McLean, Aileen Sandilands, Frances J. D. Smith, E. Birgitte Lane, Jianjun Liu, John E. A. Common

Biological Chemistry and Drug Discovery

Research output: Contribution to journal › Letter › peer-review

39 Citations (Scopus)

451 Downloads (Pure)

Abstract

Comprehensive sequencing of FLG is a challenging endeavour. We have developed a method using array-based amplicon PCR and NGS for a robust and cost efficient method to analyze this major atopic dermatitis risk factor.

Original language	English
Pages (from-to)	814-816
Number of pages	3
Journal	Journal of Allergy and Clinical Immunology
Volume	141
Issue number	2
Early online date	19 Oct 2017
DOIs	https://doi.org/10.1016/j.jaci.2017.10.001
Publication status	Published - Feb 2018

Keywords

Atopic dermatitis
Filaggrin
Mutations
Microfluidics
NGS
Skin

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/j.jaci.2017.10.001Licence: CC BY

Author Accepted ManuscriptAccepted author manuscript, 3.65 MBLicence: CC BY-NC-ND
Publisher Final Version
Copyright 2017 The Authors. Published by Elsevier Inc. on behalf of the American Academy of Allergy, Asthma&Immunology. This is an open access article under theCCBYlicense
Final published version, 562 KBLicence: CC BY

MICA: Delivering Gene Silencing Therapy to the Epidermis and Ocular Surface
Hickerson, R. (Investigator) & McLean, I. (Investigator)
Medical Research Council
1/06/17 → 31/05/24
Project: Research
Dermatology and Genetic Medicine (Strategic Grant) (Joint with Kings College London)
Barton, G. (Investigator), Campbell, P. (Investigator), Hickerson, R. (Investigator), Leigh, I. (Investigator), McLean, I. (Investigator) & Wyatt, P. (Investigator)
1/08/12 → 30/04/19
Project: Research

Cite this

Wong, X. F. C. C., Denil, S. L. I. J., Foo, J. N., Chen, H., Ling Tay, A. S., Haines, R. L., Tang, M. B. Y., McLean, W. H. I., Sandilands, A., Smith, F. J. D., Lane, E. B., Liu, J., & Common, J. E. A. (2018). Array-based sequencing of filaggrin gene for comprehensive detection of disease-associated variants. Journal of Allergy and Clinical Immunology, 141(2), 814-816. https://doi.org/10.1016/j.jaci.2017.10.001

@article{c5fd13b25e814147b83ef68cf52244a6,

title = "Array-based sequencing of filaggrin gene for comprehensive detection of disease-associated variants",

abstract = "Comprehensive sequencing of FLG is a challenging endeavour. We have developed a method using array-based amplicon PCR and NGS for a robust and cost efficient method to analyze this major atopic dermatitis risk factor.",

keywords = "Atopic dermatitis, Filaggrin, Mutations, Microfluidics, NGS, Skin",

author = "Wong, {X. F. Colin C.} and Denil, {Simon L. I. J.} and Foo, {Jia Nee} and Huijia Chen and {Ling Tay}, {Angeline Su} and Haines, {Rebecca L.} and Tang, {Mark B. Y.} and McLean, {W. H. Irwin} and Aileen Sandilands and Smith, {Frances J. D.} and Lane, {E. Birgitte} and Jianjun Liu and Common, {John E. A.}",

note = "This work was funded by A*STAR SPF grants for basic and translational skin research (IAF SPF 2013/004; IAF SPF 2013/005) to J.E.A.C., E.B.L., J.N.F., S.L.I.J.D. and J.L., and A*STAR SPF genetic orphan diseases (IAF SPF 2012/005) to X.F.C.C.W. A.S and F.D.J.S. were supported by a Wellcome Trust Strategic Award (098439) to W.H.I.M",

year = "2018",

month = feb,

doi = "10.1016/j.jaci.2017.10.001",

language = "English",

volume = "141",

pages = "814--816",

journal = "Journal of Allergy and Clinical Immunology",

issn = "0091-6749",

publisher = "Elsevier",

number = "2",

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Wong, XFCC, Denil, SLIJ, Foo, JN, Chen, H, Ling Tay, AS, Haines, RL, Tang, MBY, McLean, WHI , Sandilands, A, Smith, FJD, Lane, EB, Liu, J & Common, JEA 2018, 'Array-based sequencing of filaggrin gene for comprehensive detection of disease-associated variants', Journal of Allergy and Clinical Immunology, vol. 141, no. 2, pp. 814-816. https://doi.org/10.1016/j.jaci.2017.10.001

TY - JOUR

T1 - Array-based sequencing of filaggrin gene for comprehensive detection of disease-associated variants

AU - Wong, X. F. Colin C.

AU - Denil, Simon L. I. J.

AU - Foo, Jia Nee

AU - Chen, Huijia

AU - Ling Tay, Angeline Su

AU - Haines, Rebecca L.

AU - Tang, Mark B. Y.

AU - McLean, W. H. Irwin

AU - Sandilands, Aileen

AU - Smith, Frances J. D.

AU - Lane, E. Birgitte

AU - Liu, Jianjun

AU - Common, John E. A.

N1 - This work was funded by A*STAR SPF grants for basic and translational skin research (IAF SPF 2013/004; IAF SPF 2013/005) to J.E.A.C., E.B.L., J.N.F., S.L.I.J.D. and J.L., and A*STAR SPF genetic orphan diseases (IAF SPF 2012/005) to X.F.C.C.W. A.S and F.D.J.S. were supported by a Wellcome Trust Strategic Award (098439) to W.H.I.M

PY - 2018/2

Y1 - 2018/2

N2 - Comprehensive sequencing of FLG is a challenging endeavour. We have developed a method using array-based amplicon PCR and NGS for a robust and cost efficient method to analyze this major atopic dermatitis risk factor.

AB - Comprehensive sequencing of FLG is a challenging endeavour. We have developed a method using array-based amplicon PCR and NGS for a robust and cost efficient method to analyze this major atopic dermatitis risk factor.

KW - Atopic dermatitis

KW - Filaggrin

KW - Mutations

KW - Microfluidics

KW - NGS

KW - Skin

U2 - 10.1016/j.jaci.2017.10.001

DO - 10.1016/j.jaci.2017.10.001

M3 - Letter

C2 - 29056476

SN - 0091-6749

VL - 141

SP - 814

EP - 816

JO - Journal of Allergy and Clinical Immunology

JF - Journal of Allergy and Clinical Immunology

IS - 2

ER -

Array-based sequencing of filaggrin gene for comprehensive detection of disease-associated variants

Abstract

Keywords

ASJC Scopus subject areas

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Projects

MICA: Delivering Gene Silencing Therapy to the Epidermis and Ocular Surface

Dermatology and Genetic Medicine (Strategic Grant) (Joint with Kings College London)

Cite this