Arsenite blocks growth factor induced activation of the MAP kinase cascade, upstream of Ras and downstream of Grb2-Sos

Yair N. Doza, Clare A. Hall-Jackson, Philip Cohen (Lead / Corresponding author)

    Research output: Contribution to journalArticle

    22 Citations (Scopus)

    Abstract

    Pretreatment of cells with 0.5 mM sodium arsenite (but not other activators of stress-activated MAP kinase cascades) prevents the activation of p21Ras and strongly suppresses the activation of c-Raf and the MAP kinase cascade by a variety of growth factors. Arsenite appears to exert its effect by preventing the guanine nucleotide exchange factor mSos from converting Ras to its active GTP-bound state. Exposure to arsenite may be a simple way of assessing whether Ras plays an essential role in mediating activation of the MAP kinase cascade by extracellular signals.

    Original languageEnglish
    Pages (from-to)19-24
    Number of pages6
    JournalOncogene
    Volume17
    Issue number1
    DOIs
    Publication statusPublished - 9 Jul 1998

    Keywords

    • EGF-receptor
    • MAP kinase kinase (MKK)
    • mSos
    • P70 S6 kinase
    • Raf
    • Stress

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