Abstract
Pretreatment of cells with 0.5 mM sodium arsenite (but not other activators of stress-activated MAP kinase cascades) prevents the activation of p21Ras and strongly suppresses the activation of c-Raf and the MAP kinase cascade by a variety of growth factors. Arsenite appears to exert its effect by preventing the guanine nucleotide exchange factor mSos from converting Ras to its active GTP-bound state. Exposure to arsenite may be a simple way of assessing whether Ras plays an essential role in mediating activation of the MAP kinase cascade by extracellular signals.
Original language | English |
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Pages (from-to) | 19-24 |
Number of pages | 6 |
Journal | Oncogene |
Volume | 17 |
Issue number | 1 |
DOIs | |
Publication status | Published - 9 Jul 1998 |
Keywords
- EGF-receptor
- MAP kinase kinase (MKK)
- mSos
- P70 S6 kinase
- Raf
- Stress
ASJC Scopus subject areas
- Molecular Biology
- Genetics
- Cancer Research