Assembly of transcriptionally active RNA polymerase I initiation factor SL1 from recombinant subunits

J. C. Zomerdijk, H. Beckmann, L. Comai, R. Tjian

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    Abstract

    Initiation of ribosomal RNA synthesis by RNA polymerase I requires the promoter selec tivity factor SL1, which consists of the TATA-binding protein, TBP, and three associated factors, TAF(I)s 110, 63, and 48. Here the in vivo and in vitro assembly of functional SL1 complexes from recombinant TAF(I)s and TBP are reported. Complexes containing TBP and all three TAF(I)s were as active in supporting transcription from the human ribosomal RNA gene promoter as endogenous SL1, whereas partial complexes without TBP did not efficiently direct transcription in vitro. These results suggest that TAF(I)s 110, 63, and 48, together with TBP, are necessary and sufficient to reconstitute a transcriptionally active SL1 complex.

    Original languageEnglish
    Pages (from-to)2015-2018
    Number of pages4
    JournalScience
    Volume266
    Issue number5193
    DOIs
    Publication statusPublished - 1994

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