Assessment of Ischemic Index in Retinal Vascular Diseases Using Ultra-Wide-Field Fluorescein Angiography: Single Versus Summarized Image

Valentina Franco-Cardenas, Sanket U. Shah, David Apap, Anthony Joseph, Gad Heilweil, Kris Zutis, Emanuele Trucco, Jean Pierre Hubschman (Lead / Corresponding author)

Research output: Contribution to journalArticle

7 Citations (Scopus)
125 Downloads (Pure)


Background and Objective: To compare a single image with a computer-generated summarized image from the ultra-wide-field fluorescein angiogram (UWFFA) sequence for evaluation of ischemic index (ISI).

Materials and Methods: UWFFA sequences from patients with diabetic retinopathy (DR) (n=5), branch retinal vein occlusion (BRVO) (n=5), and central retinal vein occlusion (CRVO) (n=5) were evaluated by six graders. A single image best illustrating retinal non-perfusion was compared to a summarized image generated by computerized superimposition of angiograms. Non-perfused and ungradable retinal areas were outlined and the ISI between the single and summarized images was compared.

Results: The mean ISI in the single versus (vs) summarized images was 17% vs 15% in BRVO (p=0.12), 48% vs 48% in CRVO (p=0.67), and 25% vs 23% in DR (p=0.005). Inter-grader agreement of ISI in single versus summarized images was 0.43 vs 0.40 in BRVO, 0.69 vs 0.71 in CRVO, and 0.53 vs 0.34 in DR.

Conclusion: Computer-generated summarized images were similar to single images for grading ISI in BRVO and CRVO, but underestimated it in DR.

Original languageEnglish
Pages (from-to)353-357
Number of pages5
JournalSeminars in Ophthalmology
Early online date14 Apr 2016
Publication statusPublished - Apr 2016



  • Automated retinal image analysis
  • branch retinal vein occlusion
  • capillary dropout
  • central retinal vein occlusion
  • diabetic retinopathy
  • fluorescein angiography
  • inter-grader agreement
  • ischemic index
  • non-perfusion
  • retinal ischemia

Cite this