Assessment of ROS Production in the Mitochondria of Live Cells

Plamena R. Angelova, Albena T. Dinkova-Kostova, Andrey Y. Abramov (Lead / Corresponding author)

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Abstract

Production of reactive oxygen species (ROS) in the mitochondria plays multiple roles in physiology, and excessive production of ROS leads to the development of various pathologies. ROS in the mitochondria are generated by various enzymes, mainly in the electron transporvt chain, and it is important to identify not only the trigger but also the source of free radical production. It is important to measure mitochondrial ROS in live, intact cells, because activation of ROS production could be initiated by changes in extramitochondrial processes which could be overseen when using isolated mitochondria. Here we describe the approaches, which allow to measure production of ROS in the matrix of mitochondria in live cells. We also demonstrate how to measure kinetic changes in lipid peroxidation in mitochondria of live cells. These methods could be used for understanding the mechanisms of pathology in a variety of disease models and also for testing neuro- or cardioprotective chemicals.

Original languageEnglish
Title of host publicationReactive Oxygen Species
EditorsJesús Espada
Place of PublicationNew York
PublisherHumana Press
Pages33-42
Number of pages10
ISBN (Electronic)9781071608968
ISBN (Print)9781071608951
DOIs
Publication statusE-pub ahead of print - 29 Aug 2020

Publication series

NameMethods in Molecular Biology
Volume2202
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Lipid peroxidation
  • Live cell imaging
  • Mitochondria
  • ROS production

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  • Cite this

    Angelova, P. R., Dinkova-Kostova, A. T., & Abramov, A. Y. (2021). Assessment of ROS Production in the Mitochondria of Live Cells. In J. Espada (Ed.), Reactive Oxygen Species (pp. 33-42). (Methods in Molecular Biology; Vol. 2202). Humana Press. https://doi.org/10.1007/978-1-0716-0896-8_2