Association between a common missense variant in LOXL3 gene and the risk of non-syndromic cleft palate

Mohammad Faisal Jamal Khan, Julian Little, Peter Mossey, Regine P. M. Steegers-Theunissen, Martina Bonsi, Rita Bassi Andreasi, Michele Rubini (Lead / Corresponding author)

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7 Citations (Scopus)
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To investigate possible association between functional common variants in the lysyl oxidase like 3 gene and non-syndromic cleft palate we selected a common missense variant p.Ile615Phe (rs17010021), which was predicted to have a probably damaging effect on the lysyl oxidase like 3 enzyme. We genotyped 258 non-syndromic cleft palate case-parent triads of European origin and tested genetic association using the transmission disequilibrium test and log-linear regression analyses of genotypic relative risks and of parent-of-origin effects. The observed genotype frequency in parents was in Hardy-Weinberg equilibrium. Compared with wild-type Ile/Ile homozygotes, the relative risks for Phe/Phe homozygote infants was 6.87 (P value 3.0 × 10-3 ), while that for Ile/Phe heterozygotes was not significant. Assuming an autosomal recessive model, the relative risks for Phe/Phe genotype resulted 10.54 (P value 2.9 × 10-5 ), with a 3.6% population attributable risk. No parent-of-origin effect was observed. The identification in lysyl oxidase like 3 of a missense variant which under a recessive model associates with 10-fold increased risk of non-syndromic cleft palate supports the hypothesis that the genetic etiology of this congenital anomaly includes relatively uncommon recessive variants with moderate penetrance and located in genes which are also involved in syndromes that include cleft palate as part of the phenotype. Our findings require functional validation and replication in a larger independent genetic association study.

Original languageEnglish
Pages (from-to)136-140
Number of pages5
JournalCongenital Anomalies
Issue number4
Early online date26 May 2018
Publication statusPublished - 3 Jul 2018


  • cleft palate
  • lysyl oxidase like 3
  • missense variant
  • non-syndromic
  • Genetic Predisposition to Disease
  • Amino Acid Oxidoreductases/genetics
  • Humans
  • Genotype
  • Male
  • Mutation, Missense
  • Case-Control Studies
  • Cleft Lip/epidemiology
  • Cleft Palate/epidemiology
  • Europe/epidemiology
  • Female
  • Polymorphism, Single Nucleotide
  • Infant, Newborn

ASJC Scopus subject areas

  • General Medicine


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