Association mapping and genetic dissection of drought-induced canopy temperature differences in rice

Giovanni Melandri, Ankush Prashar, Susan R. Mccouch, Gerard Van Der Linden, Hamlyn G. Jones, Niteen Kadam, Krishna Jagadish, Harro Bouwmeester (Lead / Corresponding author), Carolien Ruyter-Spira

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    Abstract

    Drought-stressed plants display reduced stomatal conductance, which results in increased leaf temperature by limiting transpiration. In this study, thermal imaging was used to quantify the differences in canopy temperature under drought in a rice diversity panel consisting of 293 indica accessions. The population was grown under paddy field conditions and drought stress was imposed for 2 weeks at flowering. The canopy temperature of the accessions during stress negatively correlated with grain yield (r= -0.48) and positively with plant height (r=0.56). Temperature values were used to perform a genome-wide association (GWA) analysis using a 45K single nucleotide polynmorphism (SNP) map. A quantitative trait locus (QTL) for canopy temperature under drought was detected on chromosome 3 and fine-mapped using a high-density imputed SNP map. The candidate genes underlying the QTL point towards differences in the regulation of guard cell solute intake for stomatal opening as the possible source of temperature variation. Genetic variation for the significant markers of the QTL was present only within the tall, low-yielding landraces adapted to drought-prone environments. The absence of variation in the shorter genotypes, which showed lower leaf temperature and higher grain yield, suggests that breeding for high grain yield in rice under paddy conditions has reduced genetic variation for stomatal response under drought.

    Original languageEnglish
    Pages (from-to)1614-1627
    Number of pages14
    JournalJournal of Experimental Botany
    Volume71
    Issue number4
    Early online date17 Dec 2019
    DOIs
    Publication statusPublished - 7 Feb 2020

    Keywords

    • Canopy temperature
    • Oryza sativa
    • drought
    • genome-wide association studies (GWAS)
    • haplotype analysis
    • thermal imaging

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  • Cite this

    Melandri, G., Prashar, A., Mccouch, S. R., Van Der Linden, G., Jones, H. G., Kadam, N., Jagadish, K., Bouwmeester, H., & Ruyter-Spira, C. (2020). Association mapping and genetic dissection of drought-induced canopy temperature differences in rice. Journal of Experimental Botany, 71(4), 1614-1627. https://doi.org/10.1093/jxb/erz527