Association of phosphorylation of the T3 antigen with immune activation of T lymphocytes

D. Cantrell, A. A. Davies, M. Londei, M. Feldman, M. J. Crumpton

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Abstract

In human T lymphocytes the antigen receptor (Ti) is associated non-covalently on the cell surface with the invariant T3 antigen which comprises 3 chains: two glycosylated polypeptides of relative molecular mass 26,000 (Mr26K) and 21K (γ and δ) and one non-N-glycosylated polypeptide of Mr 19K (ε)1-7. The proposed function of T3 is to transduce the activation signals delivered via the antigen receptor8-13. Recently we have shown that phorbol esters, which stimulate protein kinase C, can induce phosphorylation of the γ subunit of the T3 antigen14,15. But the critical question is whether T3 phosphorylation occurs as a normal consequence of immune activation of T lymphocytes. In this respect, it has been shown that immune stimulation of murine T cells results in phosphorylation of Ti-associated polypeptides that may be the functional analogues of the human T3 antigen16,17. We have therefore monitored T3 phosphorylation after exposure of human T cells to antigen or phytohaemagglutinin (PHA). The data show that both stimuli initiate phosphorylation of the γ subunit of the T3 antigen which indicates that T3 phosphorylation is a physiological response to immune activation.

Original languageEnglish
Pages (from-to)540-542
Number of pages3
JournalNature
Volume325
Issue number6104
DOIs
Publication statusPublished - 1987

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    Cantrell, D., Davies, A. A., Londei, M., Feldman, M., & Crumpton, M. J. (1987). Association of phosphorylation of the T3 antigen with immune activation of T lymphocytes. Nature, 325(6104), 540-542. https://doi.org/10.1038/325540a0