ATP-sensitive K$^{+}$-channel run-down is Mg$^{2+}$ dependent

R. Z. Kozlowski, M. L. J. Ashford

    Research output: Contribution to journalArticle

    63 Citations (Scopus)

    Abstract

    ATP-sensitive K$^{+}$-channel currents were recorded from isolated membrane patches and voltage-clamped CRI-G1 insulin-secreting cells. Internal Mg$^{2+}$ ions inhibited ATP-K$^{+}$ channels by a voltage-dependent block of the channel current and decrease of open-state probability. The run-down of ATP-K$^{+}$ channel activity was also shown to be [Mg$^{2+}$]$_{\text{i}}$ dependent, being almost abolished in Mg$^{2+}$-free conditions. Substitution of Mn$^{2+}$ for Mg$^{2+}$ did not prevent run-down, nor did the presence of phosphate-donating nucleotides, a protease or phosphatase inhibitor or replacement of Cl$^{-}$ by gluconate.
    Original languageEnglish
    Pages (from-to)397-410
    Number of pages14
    JournalProceedings of the Royal Society of London: B. Biological Sciences
    Volume240
    Issue number1298
    DOIs
    Publication statusPublished - 22 Jun 1990

    Keywords

    • Rats
    • Animals
    • Egtazic Acid
    • Electric Conductivity
    • Kinetics
    • Membrane Potentials
    • Adenosine Triphosphate
    • Magnesium
    • Manganese
    • Potassium Channels
    • Cell Line

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