Projects per year
To establish chromosome bi-orientation, aberrant kinetochore–microtubule interaction must be resolved (error correction) by Aurora B kinase. Aurora B differentially regulates kinetochore attachment to the microtubule plus end and its lateral side (end-on and lateral attachment, respectively). However, it is still unclear how kinetochore–microtubule interactions are exchanged during error correction. Here we reconstituted the budding yeast kinetochore–microtubule interface in vitro by attaching the Ndc80 complexes to nanobeads. These Ndc80C–nanobeads recapitulated in vitro the lateral and end-on attachments of authentic kinetochores, on dynamic microtubules loaded with the Dam1 complex. This in vitro assay enabled the direct comparison of lateral and end-on attachment strength and showed that Dam1 phosphorylation by Aurora B makes the end-on attachment weaker than the lateral attachment. Similar reconstitutions with purified kinetochore particles were used for comparison. We suggest the Dam1 phosphorylation weakens interaction with the Ndc80 complex, disrupts the end-on attachment and promotes the exchange to a new lateral attachment, leading to error correction.
|Number of pages||14|
|Journal||Journal of Cell Biology|
|Early online date||14 Apr 2021|
|Publication status||Published - 7 Jun 2021|
- Cell cycle and division
ASJC Scopus subject areas
- Cell Biology
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Kinetochore-Microtubule Interactions: Steps Towards Bi-Orientation (Investigator Award)
1/12/20 → 30/11/25
Dynamics of Fundamental Cellular Processes by Live Cell and Tissue Imaging
MacDonald, M., McGloin, D., McKenna, S., Storey, K., Swedlow, J. & Weijer, K.
1/01/13 → 31/12/17
Strategic Award: Wellcome Trust Technology Platform
Blow, J., Lamond, A. & Owen-Hughes, T.
1/01/13 → 30/09/18