Author Correction: C151 in KEAP1 is the main cysteine sensor for the cyanoenone class of NRF2 activators, irrespective of molecular size or shape

Sharadha Dayalan Naidu; Aki Muramatsu; Ryota Saito; Soichiro Asami; Tadashi Honda; Tomonori Hosoya; Ken Itoh; Masayuki Yamamoto; Takafumi Suzuki; Albena T. Dinkova-Kostova

doi:10.1038/s41598-024-55265-5

Author Correction: C151 in KEAP1 is the main cysteine sensor for the cyanoenone class of NRF2 activators, irrespective of molecular size or shape

Sharadha Dayalan Naidu, Aki Muramatsu, Ryota Saito, Soichiro Asami, Tadashi Honda, Tomonori Hosoya, Ken Itoh, Masayuki Yamamoto, Takafumi Suzuki (Lead / Corresponding author), Albena T. Dinkova-Kostova (Lead / Corresponding author)

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Abstract

Correction to: Scientific Reportshttps://doi.org/10.1038/s41598-018-26269-9, published online 23 May 2018

This Article contains an error in Figure 3.

As a result of an error during the preparation of the figures for this Article, the western blots shown in Figure 3A and 3B contained an additional lane for the protein Tubulin. This is because an additional sample was loaded in the last lane of the gel to prevent potential stretching of the gel in this lane during electrophoresis if left empty. It was subsequently left uncropped from the tubulin blot shown in the published figure.

The corrected Figure 3 and its accompanying legend appear below.

C151 in KEAP1 is the primary sensor for MCE-23 and MCE-1 in MEF cells. Western blot analyses of total cell lysates of KEAP1-knockout MEF cells rescued with either wild-type (WT), single cysteine mutant C151S, double cysteine mutant C273W/C288E or triple cysteine mutant C151S/C273W/C288E of mouse N-terminally tagged HA-KEAP1. Cells (3 × 10⁵ per well), growing in 6-well plates, were exposed to vehicle (0.1% DMSO) (A,B), MCE-23 (A) or MCE-1 (B) for 3 h, after which the cells were lysed. Immunoblotting was performed on cell lysates using antibodies raised against NRF2, HA and α-tubulin.

Original language	English
Article number	4774
Number of pages	2
Journal	Scientific Reports
Volume	14
Issue number	1
DOIs	https://doi.org/10.1038/s41598-024-55265-5
Publication status	Published - 27 Feb 2024

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Dayalan Naidu, S., Muramatsu, A., Saito, R., Asami, S., Honda, T., Hosoya, T., Itoh, K., Yamamoto, M., Suzuki, T., & Dinkova-Kostova, A. T. (2024). Author Correction: C151 in KEAP1 is the main cysteine sensor for the cyanoenone class of NRF2 activators, irrespective of molecular size or shape. Scientific Reports, 14(1), Article 4774. https://doi.org/10.1038/s41598-024-55265-5

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title = "Author Correction: C151 in KEAP1 is the main cysteine sensor for the cyanoenone class of NRF2 activators, irrespective of molecular size or shape",

abstract = "Correction to: Scientific Reportshttps://doi.org/10.1038/s41598-018-26269-9, published online 23 May 2018 This Article contains an error in Figure 3.As a result of an error during the preparation of the figures for this Article, the western blots shown in Figure 3A and 3B contained an additional lane for the protein Tubulin. This is because an additional sample was loaded in the last lane of the gel to prevent potential stretching of the gel in this lane during electrophoresis if left empty. It was subsequently left uncropped from the tubulin blot shown in the published figure. The corrected Figure 3 and its accompanying legend appear below. C151 in KEAP1 is the primary sensor for MCE-23 and MCE-1 in MEF cells. Western blot analyses of total cell lysates of KEAP1-knockout MEF cells rescued with either wild-type (WT), single cysteine mutant C151S, double cysteine mutant C273W/C288E or triple cysteine mutant C151S/C273W/C288E of mouse N-terminally tagged HA-KEAP1. Cells (3 × 105 per well), growing in 6-well plates, were exposed to vehicle (0.1% DMSO) (A,B), MCE-23 (A) or MCE-1 (B) for 3 h, after which the cells were lysed. Immunoblotting was performed on cell lysates using antibodies raised against NRF2, HA and α-tubulin.",

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AU - Dayalan Naidu, Sharadha

AU - Muramatsu, Aki

AU - Saito, Ryota

AU - Asami, Soichiro

AU - Honda, Tadashi

AU - Hosoya, Tomonori

AU - Itoh, Ken

AU - Yamamoto, Masayuki

AU - Suzuki, Takafumi

AU - Dinkova-Kostova, Albena T.

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N2 - Correction to: Scientific Reportshttps://doi.org/10.1038/s41598-018-26269-9, published online 23 May 2018 This Article contains an error in Figure 3.As a result of an error during the preparation of the figures for this Article, the western blots shown in Figure 3A and 3B contained an additional lane for the protein Tubulin. This is because an additional sample was loaded in the last lane of the gel to prevent potential stretching of the gel in this lane during electrophoresis if left empty. It was subsequently left uncropped from the tubulin blot shown in the published figure. The corrected Figure 3 and its accompanying legend appear below. C151 in KEAP1 is the primary sensor for MCE-23 and MCE-1 in MEF cells. Western blot analyses of total cell lysates of KEAP1-knockout MEF cells rescued with either wild-type (WT), single cysteine mutant C151S, double cysteine mutant C273W/C288E or triple cysteine mutant C151S/C273W/C288E of mouse N-terminally tagged HA-KEAP1. Cells (3 × 105 per well), growing in 6-well plates, were exposed to vehicle (0.1% DMSO) (A,B), MCE-23 (A) or MCE-1 (B) for 3 h, after which the cells were lysed. Immunoblotting was performed on cell lysates using antibodies raised against NRF2, HA and α-tubulin.

AB - Correction to: Scientific Reportshttps://doi.org/10.1038/s41598-018-26269-9, published online 23 May 2018 This Article contains an error in Figure 3.As a result of an error during the preparation of the figures for this Article, the western blots shown in Figure 3A and 3B contained an additional lane for the protein Tubulin. This is because an additional sample was loaded in the last lane of the gel to prevent potential stretching of the gel in this lane during electrophoresis if left empty. It was subsequently left uncropped from the tubulin blot shown in the published figure. The corrected Figure 3 and its accompanying legend appear below. C151 in KEAP1 is the primary sensor for MCE-23 and MCE-1 in MEF cells. Western blot analyses of total cell lysates of KEAP1-knockout MEF cells rescued with either wild-type (WT), single cysteine mutant C151S, double cysteine mutant C273W/C288E or triple cysteine mutant C151S/C273W/C288E of mouse N-terminally tagged HA-KEAP1. Cells (3 × 105 per well), growing in 6-well plates, were exposed to vehicle (0.1% DMSO) (A,B), MCE-23 (A) or MCE-1 (B) for 3 h, after which the cells were lysed. Immunoblotting was performed on cell lysates using antibodies raised against NRF2, HA and α-tubulin.

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Author Correction: C151 in KEAP1 is the main cysteine sensor for the cyanoenone class of NRF2 activators, irrespective of molecular size or shape

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