Automated design of ligands to polypharmacological profiles

Jeremy Besnard, Gian Filippo Ruda, Vincent Setola, Keren Abecassis, Ramona M. Rodriguiz, Xi-Ping Huang, Suzanne Norval, Maria F. Sassano, Antony I. Shin, Lauren A. Webster, Frederick R. C. Simeons, Laste Stojanovski, Annik Prat, Nabil G. Seidah, Daniel B. Constam, G. Richard Bickerton, Kevin D. Read, William C. Wetsel, Ian H. Gilbert, Bryan L. Roth (Lead / Corresponding author)Andrew L. Hopkins

    Research output: Contribution to journalArticle

    407 Citations (Scopus)

    Abstract

    The clinical efficacy and safety of a drug is determined by its activity profile across many proteins in the proteome. However, designing drugs with a specific multi-target profile is both complex and difficult. Therefore methods to design drugs rationally a priori against profiles of several proteins would have immense value in drug discovery. Here we describe a new approach for the automated design of ligands against profiles of multiple drug targets. The method is demonstrated by the evolution of an approved acetylcholinesterase inhibitor drug into brain-penetrable ligands with either specific polypharmacology or exquisite selectivity profiles for G-protein-coupled receptors. Overall, 800 ligand-target predictions of prospectively designed ligands were tested experimentally, of which 75% were confirmed to be correct. We also demonstrate target engagement in vivo. The approach can be a useful source of drug leads when multi-target profiles are required to achieve either selectivity over other drug targets or a desired polypharmacology.

    Original languageEnglish
    Pages (from-to)215-+
    Number of pages8
    JournalNature
    Volume492
    Issue number7428
    DOIs
    Publication statusPublished - 13 Dec 2012

    Press / Media

    Automated design for Drug Discovery

    Andrew L. Hopkins, Suzanne Norval, Jeremy Besnard, Laste Stojanovski, Richard Bickerton, Kevin Read & Ian Gilbert

    13/12/12

    1 item of Media coverage

    Press/Media: Research

    Cite this

    Besnard, J., Ruda, G. F., Setola, V., Abecassis, K., Rodriguiz, R. M., Huang, X-P., Norval, S., Sassano, M. F., Shin, A. I., Webster, L. A., Simeons, F. R. C., Stojanovski, L., Prat, A., Seidah, N. G., Constam, D. B., Bickerton, G. R., Read, K. D., Wetsel, W. C., Gilbert, I. H., ... Hopkins, A. L. (2012). Automated design of ligands to polypharmacological profiles. Nature, 492(7428), 215-+. https://doi.org/10.1038/nature11691