Automated laser guidance of neuronal growth cones using a spatial light modulator

David J. Carnegie, Tomas Cizmar, Jorg Baumgartl, Frank J. Gunn-Moore, Kishan Dholakia

    Research output: Contribution to journalArticlepeer-review

    17 Citations (Scopus)

    Abstract

    The growth cone of a developing neuron can be guided using a focused infra-red (IR) laser beam [1]. In previous setups this process has required a significant amount of user intervention to adjust continuously the laser beam to guide the growing neuron. Previously, a system using an acousto-optical deflector (AOD) has been developed to steer the beam [2]. However, to enhance the controllability of this system, here we demonstrate the use of a computer controlled spatial light modulator (SLM) to steer and manipulate the shape of a laser beam for use in guided neuronal growth. This new experimental setup paves the way to enable a comprehensive investigation into beam shaping effects on neuronal growth and we show neuronal growth initiated by a Bessel light mode. This is a robust platform to explore the biochemistry of this novel phenomenon.

    Original languageEnglish
    Pages (from-to)682-692
    Number of pages11
    JournalJournal of Biophotonics
    Volume2
    Issue number11
    DOIs
    Publication statusPublished - Nov 2009

    Keywords

    • neuron growth
    • MICROMANIPULATION
    • HOLOGRAPHIC OPTICAL TWEEZERS
    • TRANSFECTION
    • CELLS
    • MANIPULATION
    • COMPUTER-GENERATED HOLOGRAMS
    • Bessel beam
    • FEMTOSECOND LASER
    • PARTICLES
    • spatial light modulator
    • MICROSCOPY
    • TRAP

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