Autoregulation of FCA pre-mRNA processing controls Arabidopsis flowering time

Victor Quesada, Richard Macknight, Caroline Dean, Gordon G. Simpson

    Research output: Contribution to journalArticle

    204 Citations (Scopus)

    Abstract

    The timing of the transition to flowering is critical for reproductive success in plants. Arabidopsis FCA encodes an RNA-binding protein that promotes flowering. FCA expression is regulated through alternative processing of its pre-mRNA. We demonstrate here that FCA negatively regulates its own expression by ultimately promoting cleavage and polyadenylation within intron 3. This causes the production of a truncated, inactive transcript at the expense of the full-length FCA mRNA, thus limiting the expression of active FCA protein. We show that this negative autoregulation is under developmental control and requires the FCA WW protein interaction domain. Removal of introns from FCA bypasses the autoregulation, and the resulting increased levels of FCA protein overcomes the repression of flowering normally conferred through the up-regulation of FLC by active FRI alleles. The negative autoregulation of FCA may therefore have evolved to limit FCA activity and hence control flowering time.
    Original languageEnglish
    Pages (from-to)3142-3152
    Number of pages11
    JournalThe EMBO Journal
    Volume22
    Issue number12
    DOIs
    Publication statusPublished - Jan 2003

    Keywords

    • Arabidopsis
    • FCA
    • Polydenylation

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