Autoregulatory control of the p53 response by Siah-1L-mediated HIPK2 degradation

Marco A. Calzado; Laureano de la Vega; Eduardo Muñoz; M. Lienhard Schmitz

doi:10.1515/BC.2009.112

Autoregulatory control of the p53 response by Siah-1L-mediated HIPK2 degradation

Marco A. Calzado, Laureano de la Vega, Eduardo Muñoz, M. Lienhard Schmitz (Lead / Corresponding author)

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

The different activities of the tumor suppressor p53 are tightly regulated by various negative and positive feedback loops, which allow accurate control of its function. Here we show that the p53-inducible ubiquitin E3 ligase Siah-1L can bind to the p53 phosphorylating kinase HIPK2 and thus allows its ubiquitination and proteasomal elimination. Siah-1L also eliminates the HIPK family member HIPK3, indicating that its activity is not restricted to one member of the HIPK family. The stimulating effect of HIPK2 on p53-triggered transcription is counteracted by Siah-1L, thus showing the occurrence of another negative feedback loop controlling the p53 response.

Original language	English
Pages (from-to)	1079-1083
Number of pages	5
Journal	Biological Chemistry
Volume	390
Issue number	10
Early online date	30 Jul 2009
DOIs	https://doi.org/10.1515/BC.2009.112
Publication status	Published - Oct 2009

Keywords

Apoptosis
Base sequence
Carrier proteins
Cell line
Down-regulation
Humans
Nuclear proteins
Polyubiquitin
Protein-Serine-Threonine Kinases
Transcription, Genetic
Tumor suppressor protein p53
Ubiquitin-Protein Ligases
Up-regulation

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title = "Autoregulatory control of the p53 response by Siah-1L-mediated HIPK2 degradation",

abstract = "The different activities of the tumor suppressor p53 are tightly regulated by various negative and positive feedback loops, which allow accurate control of its function. Here we show that the p53-inducible ubiquitin E3 ligase Siah-1L can bind to the p53 phosphorylating kinase HIPK2 and thus allows its ubiquitination and proteasomal elimination. Siah-1L also eliminates the HIPK family member HIPK3, indicating that its activity is not restricted to one member of the HIPK family. The stimulating effect of HIPK2 on p53-triggered transcription is counteracted by Siah-1L, thus showing the occurrence of another negative feedback loop controlling the p53 response.",

keywords = "Apoptosis, Base sequence, Carrier proteins, Cell line, Down-regulation, Humans, Nuclear proteins, Polyubiquitin, Protein-Serine-Threonine Kinases, Transcription, Genetic, Tumor suppressor protein p53, Ubiquitin-Protein Ligases, Up-regulation",

author = "Calzado, {Marco A.} and {de la Vega}, Laureano and Eduardo Mu{\~n}oz and Schmitz, {M. Lienhard}",

year = "2009",

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doi = "10.1515/BC.2009.112",

language = "English",

volume = "390",

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journal = "Biological Chemistry",

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T1 - Autoregulatory control of the p53 response by Siah-1L-mediated HIPK2 degradation

AU - Calzado, Marco A.

AU - de la Vega, Laureano

AU - Muñoz, Eduardo

AU - Schmitz, M. Lienhard

PY - 2009/10

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AB - The different activities of the tumor suppressor p53 are tightly regulated by various negative and positive feedback loops, which allow accurate control of its function. Here we show that the p53-inducible ubiquitin E3 ligase Siah-1L can bind to the p53 phosphorylating kinase HIPK2 and thus allows its ubiquitination and proteasomal elimination. Siah-1L also eliminates the HIPK family member HIPK3, indicating that its activity is not restricted to one member of the HIPK family. The stimulating effect of HIPK2 on p53-triggered transcription is counteracted by Siah-1L, thus showing the occurrence of another negative feedback loop controlling the p53 response.

KW - Apoptosis

KW - Base sequence

KW - Carrier proteins

KW - Cell line

KW - Down-regulation

KW - Humans

KW - Nuclear proteins

KW - Polyubiquitin

KW - Protein-Serine-Threonine Kinases

KW - Transcription, Genetic

KW - Tumor suppressor protein p53

KW - Ubiquitin-Protein Ligases

KW - Up-regulation

U2 - 10.1515/BC.2009.112

DO - 10.1515/BC.2009.112

M3 - Article

C2 - 19642869

SN - 1437-4315

VL - 390

SP - 1079

EP - 1083

JO - Biological Chemistry

JF - Biological Chemistry

IS - 10

ER -

Autoregulatory control of the p53 response by Siah-1L-mediated HIPK2 degradation

Abstract

Keywords

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