AVR2 targets BSL family members, which act as susceptibility factors to suppress host immunity

Dionne Turnbull, Haixia Wang, Susan Breen, Marek Malec, Shaista Naqvi, Lina Yang, Lydia Welsh, Piers A. Hemsley, Tian Zhendong, Frederic Brunner, Eleanor M. Gilroy, Paul R. J. Birch (Lead / Corresponding author)

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Abstract

To be successful plant pathogens, microbes use "effector proteins" to manipulate host functions to their benefit. Identifying host targets of effector proteins and characterizing their role in the infection process allow us to better understand plant-pathogen interactions and the plant immune system. Yeast two-hybrid analysis and coimmunoprecipitation were used to demonstrate that the Phytophthora infestans effector AVIRULENCE 2 (PiAVR2) interacts with all three BRI1-SUPPRESSOR1-like (BSL) family members from potato (Solanum tuberosum). Transient expression of BSL1, BSL2, and BSL3 enhanced P. infestans leaf infection. BSL1 and BSL3 suppressed INFESTIN 1 elicitin-triggered cell death, showing that they negatively regulate immunity. Virus-induced gene silencing studies revealed that BSL2 and BSL3 are required for BSL1 stability and show that basal levels of immunity are increased in BSL-silenced plants. Immune suppression by BSL family members is dependent on the brassinosteroid-responsive host transcription factor CIB1/HBI1-like 1. The P. infestans effector PiAVR2 targets all three BSL family members in the crop plant S. tuberosum These phosphatases, known for their role in growth-promoting brassinosteroid signaling, all support P. infestans virulence and thus can be regarded as susceptibility factors in late blight infection.

Original languageEnglish
Pages (from-to)571-581
Number of pages11
JournalPlant Physiology
Volume180
Issue number1
Early online date19 Feb 2019
DOIs
Publication statusPublished - 1 May 2019

Fingerprint

Phytophthora infestans
Immunity
immunity
Solanum tuberosum
Brassinosteroids
brassinosteroids
plant pathogens
Infection
Plant Immunity
infection
Gene Silencing
two hybrid system techniques
Phosphoric Monoester Hydrolases
gene silencing
Virulence
Proteins
Cell Death
Transcription Factors
Yeasts
immune system

Keywords

  • Plant immunity
  • Effector-triggered susceptibility
  • Plant disease
  • Lat blight
  • Virulence
  • Plant pathogens
  • Pathogenicity

Cite this

Turnbull, Dionne ; Wang, Haixia ; Breen, Susan ; Malec, Marek ; Naqvi, Shaista ; Yang, Lina ; Welsh, Lydia ; Hemsley, Piers A. ; Zhendong, Tian ; Brunner, Frederic ; Gilroy, Eleanor M. ; Birch, Paul R. J. / AVR2 targets BSL family members, which act as susceptibility factors to suppress host immunity. In: Plant Physiology. 2019 ; Vol. 180, No. 1. pp. 571-581.
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Turnbull, D, Wang, H, Breen, S, Malec, M, Naqvi, S, Yang, L, Welsh, L, Hemsley, PA, Zhendong, T, Brunner, F, Gilroy, EM & Birch, PRJ 2019, 'AVR2 targets BSL family members, which act as susceptibility factors to suppress host immunity', Plant Physiology, vol. 180, no. 1, pp. 571-581. https://doi.org/10.1104/pp.18.01143

AVR2 targets BSL family members, which act as susceptibility factors to suppress host immunity. / Turnbull, Dionne; Wang, Haixia; Breen, Susan; Malec, Marek; Naqvi, Shaista; Yang, Lina; Welsh, Lydia; Hemsley, Piers A.; Zhendong, Tian; Brunner, Frederic; Gilroy, Eleanor M.; Birch, Paul R. J. (Lead / Corresponding author).

In: Plant Physiology, Vol. 180, No. 1, 01.05.2019, p. 571-581.

Research output: Contribution to journalArticle

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AU - Turnbull, Dionne

AU - Wang, Haixia

AU - Breen, Susan

AU - Malec, Marek

AU - Naqvi, Shaista

AU - Yang, Lina

AU - Welsh, Lydia

AU - Hemsley, Piers A.

AU - Zhendong, Tian

AU - Brunner, Frederic

AU - Gilroy, Eleanor M.

AU - Birch, Paul R. J.

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