Projects per year
Abstract
In flowering plants, successful germinal cell development and meiotic recombination depend upon a combination of environmental and genetic factors. To gain insights into this specialized reproductive development program we used short- and long-read RNA-sequencing (RNA-seq) to study the temporal dynamics of transcript abundance in immuno-cytologically staged barley (Hordeum vulgare) anthers and meiocytes. We show that the most significant transcriptional changes in anthers occur at the transition from pre-meiosis to leptotene-zygotene, which is followed by increasingly stable transcript abundance throughout prophase I into metaphase I-tetrad. Our analysis reveals that the pre-meiotic anthers are enriched in long non-coding RNAs (lncRNAs) and that entry to meiosis is characterized by their robust and significant down regulation. Intriguingly, only 24% of a collection of putative meiotic gene orthologs showed differential transcript abundance in at least one stage or tissue comparison. Argonautes, E3 ubiquitin ligases, and lys48 specific de-ubiquitinating enzymes were enriched in prophase I meiocyte samples. These developmental, time-resolved transcriptomes demonstrate remarkable stability in transcript abundance in meiocytes throughout prophase I after the initial and substantial reprogramming at meiosis entry and the complexity of the regulatory networks involved in early meiotic processes.
Original language | English |
---|---|
Article number | 619404 |
Number of pages | 24 |
Journal | Frontiers in Plant Science |
Volume | 11 |
DOIs | |
Publication status | Published - 12 Jan 2021 |
Keywords
- barley
- anther
- meiocyte
- transcriptome
- meiosis
- argonaute
- IncRNAs
- ubiquitin
- lncRNAs
ASJC Scopus subject areas
- Plant Science
Fingerprint
Dive into the research topics of 'Barley Anther and Meiocyte Transcriptome Dynamics in Meiotic Prophase I'. Together they form a unique fingerprint.Projects
- 1 Finished
-
A Reference Transcript Database for Improved Analysis of RNA-seq Data from Barley
Brown, J. (Investigator) & Waugh, R. (Investigator)
Biotechnology and Biological Sciences Research Council
1/10/18 → 30/09/20
Project: Research