Barley root exudates collection and primary metabolite profiling

Carmen Escudero-Martinez, Alexandre Foito, Rumana Kapadia, Alessio Aprile, Davide Bulgarelli

Research output: Working paper/PreprintPreprint


Plant exudates are one of the main drivers for the proliferation of the rhizosphere microbiota. We developed a protocol to quantify primary metabolites in exudates of the global crop barley. We used 2- and 3-week-old barley plants grown in sterilised sand and their root exudates were collected for 6 hours in ddH2O water to avoid degradation/transformation of the compounds. These samples were used to determine total carbon and total nitrogen by Dumas. Finally, we profiled primary metabolites in collected exudates by gas chromatography/mass spectrometry (GC-MS).
Original languageEnglish
PublisherResearch Square
Number of pages12
Publication statusPublished - 17 Jun 2022


  • Biological techniques
  • Plant sciences
  • Analytical chemistry
  • Root exudates
  • microbiome
  • rhizosphere
  • root
  • metabolite
  • barley
  • GC/MS


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