Barley root exudates collection and primary metabolite profiling

Carmen Escudero-Martinez; Alexandre Foito; Rumana Kapadia; Alessio Aprile; Davide Bulgarelli

doi:10.21203/rs.3.pex-1900/v1

Barley root exudates collection and primary metabolite profiling

Carmen Escudero-Martinez
, Alexandre Foito
, Rumana Kapadia
, Alessio Aprile
, Davide Bulgarelli

Plant Sciences

Research output: Working paper/Preprint › Preprint

Abstract

Plant exudates are one of the main drivers for the proliferation of the rhizosphere microbiota. We developed a protocol to quantify primary metabolites in exudates of the global crop barley. We used 2- and 3-week-old barley plants grown in sterilised sand and their root exudates were collected for 6 hours in ddH2O water to avoid degradation/transformation of the compounds. These samples were used to determine total carbon and total nitrogen by Dumas. Finally, we profiled primary metabolites in collected exudates by gas chromatography/mass spectrometry (GC-MS).

Original language	English
Publisher	Research Square
Pages	1-12
Number of pages	12
DOIs	https://doi.org/10.21203/rs.3.pex-1900/v1
Publication status	Published - 17 Jun 2022

Keywords

Biological techniques
Plant sciences
Analytical chemistry
Root exudates
microbiome
rhizosphere
root
metabolite
barley
GC/MS

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10.21203/rs.3.pex-1900/v1Licence: CC BY

1 Article

Identifying plant genes shaping microbiota composition in the barley rhizosphere
Escudero-Martinez, C., Coulter, M., Alegria Terrazas, R., Foito, A., Kapadia, R., Pietrangelo, L., Maver, M., Sharma, R., Aprile, A., Morris, J. A., Hedley, P. E., Maurer, A., Pillen, K., Naclerio, G., Mimmo, T., Barton, G. J., Waugh, R., Abbott, J. & Bulgarelli, D. (Lead / Corresponding author), 16 Jun 2022, In: Nature Communications. 13, 14 p., 3443 .
Research output: Contribution to journal › Article › peer-review

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title = "Barley root exudates collection and primary metabolite profiling",

abstract = "Plant exudates are one of the main drivers for the proliferation of the rhizosphere microbiota. We developed a protocol to quantify primary metabolites in exudates of the global crop barley. We used 2- and 3-week-old barley plants grown in sterilised sand and their root exudates were collected for 6 hours in ddH2O water to avoid degradation/transformation of the compounds. These samples were used to determine total carbon and total nitrogen by Dumas. Finally, we profiled primary metabolites in collected exudates by gas chromatography/mass spectrometry (GC-MS).",

keywords = "Biological techniques, Plant sciences, Analytical chemistry, Root exudates, microbiome, rhizosphere, root, metabolite, barley, GC/MS",

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AU - Foito, Alexandre

AU - Kapadia, Rumana

AU - Aprile, Alessio

AU - Bulgarelli, Davide

PY - 2022/6/17

Y1 - 2022/6/17

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AB - Plant exudates are one of the main drivers for the proliferation of the rhizosphere microbiota. We developed a protocol to quantify primary metabolites in exudates of the global crop barley. We used 2- and 3-week-old barley plants grown in sterilised sand and their root exudates were collected for 6 hours in ddH2O water to avoid degradation/transformation of the compounds. These samples were used to determine total carbon and total nitrogen by Dumas. Finally, we profiled primary metabolites in collected exudates by gas chromatography/mass spectrometry (GC-MS).

KW - Biological techniques

KW - Plant sciences

KW - Analytical chemistry

KW - Root exudates

KW - microbiome

KW - rhizosphere

KW - root

KW - metabolite

KW - barley

KW - GC/MS

U2 - 10.21203/rs.3.pex-1900/v1

DO - 10.21203/rs.3.pex-1900/v1

M3 - Preprint

SP - 1

EP - 12

BT - Barley root exudates collection and primary metabolite profiling

PB - Research Square

ER -

Barley root exudates collection and primary metabolite profiling

Abstract

Keywords

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Research output

Identifying plant genes shaping microbiota composition in the barley rhizosphere

Cite this