Barley sodium content is regulated by natural variants of the Na+ transporter HvHKT1;5

Kelly Houston; Jiaen Qiu; Stefanie Wege; Maria Hrmova; Helena Oakey; Yue Qu; Pauline Smith; Apriadi Situmorang; Malcolm Macaulay; Paulina Flis; Micha Bayer; Stuart Roy; Claire Halpin; Joanne Russell; Miriam Schreiber; Caitlin Byrt; Matt Gilliham; David E. Salt; Robbie Waugh

doi:10.1038/s42003-020-0990-5

Barley sodium content is regulated by natural variants of the Na+ transporter HvHKT1;5

Kelly Houston, Jiaen Qiu, Stefanie Wege, Maria Hrmova, Helena Oakey, Yue Qu, Pauline Smith, Apriadi Situmorang, Malcolm Macaulay, Paulina Flis, Micha Bayer, Stuart Roy, Claire Halpin, Joanne Russell, Miriam Schreiber, Caitlin Byrt, Matt Gilliham (Lead / Corresponding author), David E. Salt (Lead / Corresponding author), Robbie Waugh (Lead / Corresponding author)

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Abstract

During plant growth, sodium (Na+) in the soil is transported via the xylem from the root to the shoot. While excess Na+ is toxic to most plants, non-toxic concentrations have been shown to improve crop yields under certain conditions, such as when soil K+ is low. We quantified grain Na+ across a barley genome-wide association study panel grown under non-saline conditions and identified variants of a Class 1 HIGH-AFFINITY-POTASSIUM-TRANSPORTER (HvHKT1;5)-encoding gene responsible for Na+ content variation under these conditions. A leucine to proline substitution at position 189 (L189P) in HvHKT1;5 disturbs its characteristic plasma membrane localisation and disrupts Na+ transport. Under low and moderate soil Na+, genotypes containing HvHKT1:5P189 accumulate high concentrations of Na+ but exhibit no evidence of toxicity. As the frequency of HvHKT1:5P189 increases significantly in cultivated European germplasm, we cautiously speculate that this non-functional variant may enhance yield potential in non-saline environments, possibly by offsetting limitations of low available K+.

Original language	English
Article number	258
Pages (from-to)	1-9
Number of pages	9
Journal	Communications Biology
Volume	3
Issue number	1
Early online date	22 May 2020
DOIs	https://doi.org/10.1038/s42003-020-0990-5
Publication status	Published - Dec 2020

Keywords

Abiotic
Agricultural genetics
Plant genetics
Salt

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Houston, K., Qiu, J., Wege, S., Hrmova, M., Oakey, H., Qu, Y., Smith, P., Situmorang, A., Macaulay, M., Flis, P., Bayer, M., Roy, S., Halpin, C., Russell, J., Schreiber, M., Byrt, C., Gilliham, M., Salt, D. E., & Waugh, R. (2020). Barley sodium content is regulated by natural variants of the Na+ transporter HvHKT1;5. Communications Biology, 3(1), 1-9. [258]. https://doi.org/10.1038/s42003-020-0990-5

Houston, Kelly ; Qiu, Jiaen ; Wege, Stefanie ; Hrmova, Maria ; Oakey, Helena ; Qu, Yue ; Smith, Pauline ; Situmorang, Apriadi ; Macaulay, Malcolm ; Flis, Paulina ; Bayer, Micha ; Roy, Stuart ; Halpin, Claire ; Russell, Joanne ; Schreiber, Miriam ; Byrt, Caitlin ; Gilliham, Matt ; Salt, David E. ; Waugh, Robbie. / Barley sodium content is regulated by natural variants of the Na+ transporter HvHKT1;5. In: Communications Biology. 2020 ; Vol. 3, No. 1. pp. 1-9.

@article{426b39f481fe48a2a84dee355ab531a1,

title = "Barley sodium content is regulated by natural variants of the Na+ transporter HvHKT1;5",

abstract = "During plant growth, sodium (Na+) in the soil is transported via the xylem from the root to the shoot. While excess Na+ is toxic to most plants, non-toxic concentrations have been shown to improve crop yields under certain conditions, such as when soil K+ is low. We quantified grain Na+ across a barley genome-wide association study panel grown under non-saline conditions and identified variants of a Class 1 HIGH-AFFINITY-POTASSIUM-TRANSPORTER (HvHKT1;5)-encoding gene responsible for Na+ content variation under these conditions. A leucine to proline substitution at position 189 (L189P) in HvHKT1;5 disturbs its characteristic plasma membrane localisation and disrupts Na+ transport. Under low and moderate soil Na+, genotypes containing HvHKT1:5P189 accumulate high concentrations of Na+ but exhibit no evidence of toxicity. As the frequency of HvHKT1:5P189 increases significantly in cultivated European germplasm, we cautiously speculate that this non-functional variant may enhance yield potential in non-saline environments, possibly by offsetting limitations of low available K+.",

keywords = "Abiotic, Agricultural genetics, Plant genetics, Salt",

author = "Kelly Houston and Jiaen Qiu and Stefanie Wege and Maria Hrmova and Helena Oakey and Yue Qu and Pauline Smith and Apriadi Situmorang and Malcolm Macaulay and Paulina Flis and Micha Bayer and Stuart Roy and Claire Halpin and Joanne Russell and Miriam Schreiber and Caitlin Byrt and Matt Gilliham and Salt, {David E.} and Robbie Waugh",

note = "R.W., M.S., and M.M. acknowledge support from ERC project 669182 {\textquoteleft}SHUFFLE{\textquoteright} to R.W. K.H., P.S., M.B., J.R., and R.W. acknowledge the Rural & Environment Science & Analytical Services Division of the Scottish Government. C.B., J.Q., and Y.Q. acknowledge support from Rutherford Fund Strategic Partner Grants 2018 - Award Reference: RF-2018-30 to C.H. and R.W. S.R. is grateful for financial assistance from The Australian Research Council Industrial Transformation Research Hub for Wheat in a Hot and Dry Climate (IH130200027), the Grains Research and Development Corporation (ACP00009) and The Waite Research Institute, University of Adelaide. C.B. acknowledges support from the Grains Research and Development Corporation (GRDC) and the Australian Research Council (FT180100476). We are thankful to the Australian Research Council for funding through CE140100008 to M.G. (J.Q. and Y.Q.), FT180100476 to C.S.B. and DE160100804 to S.W. (A.S.). D.E.S. acknowledges support from BBSRC Grant BB/L000113/1. MH acknowledges financial support from the Huaiyin Normal University, China.",

year = "2020",

month = dec,

doi = "10.1038/s42003-020-0990-5",

language = "English",

volume = "3",

pages = "1--9",

journal = "Communications Biology",

issn = "2399-3642",

publisher = "Springer Nature",

number = "1",

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Houston, K, Qiu, J, Wege, S, Hrmova, M, Oakey, H, Qu, Y, Smith, P, Situmorang, A, Macaulay, M, Flis, P, Bayer, M, Roy, S, Halpin, C, Russell, J, Schreiber, M, Byrt, C, Gilliham, M, Salt, DE & Waugh, R 2020, 'Barley sodium content is regulated by natural variants of the Na+ transporter HvHKT1;5', Communications Biology, vol. 3, no. 1, 258, pp. 1-9. https://doi.org/10.1038/s42003-020-0990-5

Barley sodium content is regulated by natural variants of the Na+ transporter HvHKT1;5. / Houston, Kelly; Qiu, Jiaen; Wege, Stefanie; Hrmova, Maria; Oakey, Helena; Qu, Yue; Smith, Pauline; Situmorang, Apriadi; Macaulay, Malcolm; Flis, Paulina; Bayer, Micha; Roy, Stuart; Halpin, Claire; Russell, Joanne; Schreiber, Miriam; Byrt, Caitlin; Gilliham, Matt (Lead / Corresponding author); Salt, David E. (Lead / Corresponding author); Waugh, Robbie (Lead / Corresponding author).

In: Communications Biology, Vol. 3, No. 1, 258, 12.2020, p. 1-9.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Barley sodium content is regulated by natural variants of the Na+ transporter HvHKT1;5

AU - Houston, Kelly

AU - Qiu, Jiaen

AU - Wege, Stefanie

AU - Hrmova, Maria

AU - Oakey, Helena

AU - Qu, Yue

AU - Smith, Pauline

AU - Situmorang, Apriadi

AU - Macaulay, Malcolm

AU - Flis, Paulina

AU - Bayer, Micha

AU - Roy, Stuart

AU - Halpin, Claire

AU - Russell, Joanne

AU - Schreiber, Miriam

AU - Byrt, Caitlin

AU - Gilliham, Matt

AU - Salt, David E.

AU - Waugh, Robbie

N1 - R.W., M.S., and M.M. acknowledge support from ERC project 669182 ‘SHUFFLE’ to R.W. K.H., P.S., M.B., J.R., and R.W. acknowledge the Rural & Environment Science & Analytical Services Division of the Scottish Government. C.B., J.Q., and Y.Q. acknowledge support from Rutherford Fund Strategic Partner Grants 2018 - Award Reference: RF-2018-30 to C.H. and R.W. S.R. is grateful for financial assistance from The Australian Research Council Industrial Transformation Research Hub for Wheat in a Hot and Dry Climate (IH130200027), the Grains Research and Development Corporation (ACP00009) and The Waite Research Institute, University of Adelaide. C.B. acknowledges support from the Grains Research and Development Corporation (GRDC) and the Australian Research Council (FT180100476). We are thankful to the Australian Research Council for funding through CE140100008 to M.G. (J.Q. and Y.Q.), FT180100476 to C.S.B. and DE160100804 to S.W. (A.S.). D.E.S. acknowledges support from BBSRC Grant BB/L000113/1. MH acknowledges financial support from the Huaiyin Normal University, China.

PY - 2020/12

Y1 - 2020/12

N2 - During plant growth, sodium (Na+) in the soil is transported via the xylem from the root to the shoot. While excess Na+ is toxic to most plants, non-toxic concentrations have been shown to improve crop yields under certain conditions, such as when soil K+ is low. We quantified grain Na+ across a barley genome-wide association study panel grown under non-saline conditions and identified variants of a Class 1 HIGH-AFFINITY-POTASSIUM-TRANSPORTER (HvHKT1;5)-encoding gene responsible for Na+ content variation under these conditions. A leucine to proline substitution at position 189 (L189P) in HvHKT1;5 disturbs its characteristic plasma membrane localisation and disrupts Na+ transport. Under low and moderate soil Na+, genotypes containing HvHKT1:5P189 accumulate high concentrations of Na+ but exhibit no evidence of toxicity. As the frequency of HvHKT1:5P189 increases significantly in cultivated European germplasm, we cautiously speculate that this non-functional variant may enhance yield potential in non-saline environments, possibly by offsetting limitations of low available K+.

AB - During plant growth, sodium (Na+) in the soil is transported via the xylem from the root to the shoot. While excess Na+ is toxic to most plants, non-toxic concentrations have been shown to improve crop yields under certain conditions, such as when soil K+ is low. We quantified grain Na+ across a barley genome-wide association study panel grown under non-saline conditions and identified variants of a Class 1 HIGH-AFFINITY-POTASSIUM-TRANSPORTER (HvHKT1;5)-encoding gene responsible for Na+ content variation under these conditions. A leucine to proline substitution at position 189 (L189P) in HvHKT1;5 disturbs its characteristic plasma membrane localisation and disrupts Na+ transport. Under low and moderate soil Na+, genotypes containing HvHKT1:5P189 accumulate high concentrations of Na+ but exhibit no evidence of toxicity. As the frequency of HvHKT1:5P189 increases significantly in cultivated European germplasm, we cautiously speculate that this non-functional variant may enhance yield potential in non-saline environments, possibly by offsetting limitations of low available K+.

KW - Abiotic

KW - Agricultural genetics

KW - Plant genetics

KW - Salt

UR - http://www.scopus.com/inward/record.url?scp=85085263863&partnerID=8YFLogxK

U2 - 10.1038/s42003-020-0990-5

DO - 10.1038/s42003-020-0990-5

M3 - Article

C2 - 32444849

VL - 3

SP - 1

EP - 9

JO - Communications Biology

JF - Communications Biology

SN - 2399-3642

IS - 1

M1 - 258

ER -

Barley sodium content is regulated by natural variants of the Na+ transporter HvHKT1;5

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