Abstract
Chronic cannabinoid exposure during adolescence in male rats induces chronic cognitive and emotional impairments. However, the impact of this form of exposure on offspring vulnerability to stress is unknown. The aim of this study was to evaluate the behavioural and epigenetic effects of stress in the offspring of male rats whose fathers were exposed to cannabinoids during adolescence. Male adolescent offspring of Win55,212-2 (1.2 mg/kg) treated rats were exposed during one week to variable stressors and subjected to behavioural tests of anxiety and episodic-like memory, followed by an assessment of global DNA methylation and expression of DNA methyltransferases enzymes DNMT1 and DNMT3a mRNA in the prefrontal cortex. Stress exposure induced a significant anxiogenic-like effect but did not affect the episodic-like memory in the offspring of Win55,212-2 exposed fathers in comparison to the offspring of non-exposed fathers. These behavioural changes were subsequent to a significant increase in global DNA methylation and DNMT1 and DNMTa3 transcription in the prefrontal cortex. These data suggest that the deleterious effect of chronic exposure to cannabinoids during adolescence are not limited to the exposed individuals but may increase the vulnerability to stress-induced anxiety in the offspring and alter their epigenetic programming.
| Original language | English |
|---|---|
| Pages (from-to) | 48-54 |
| Number of pages | 7 |
| Journal | International Journal of Developmental Neuroscience |
| Volume | 72 |
| Early online date | 23 Nov 2018 |
| DOIs | |
| Publication status | Published - Feb 2019 |
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Keywords
- Adolescence
- Anxiety
- Cannabinoids
- DNA methylation
- Offspring
- Stress
Cite this
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Behavioural and epigenetic effects of paternal exposure to cannabinoids during adolescence on offspring vulnerability to stress. / Andaloussi, Zineb Ibn Lahmar; Taghzouti, Khalid; Abboussi, Oualid (Lead / Corresponding author).
In: International Journal of Developmental Neuroscience, Vol. 72, 02.2019, p. 48-54.Research output: Contribution to journal › Article
TY - JOUR
T1 - Behavioural and epigenetic effects of paternal exposure to cannabinoids during adolescence on offspring vulnerability to stress
AU - Andaloussi, Zineb Ibn Lahmar
AU - Taghzouti, Khalid
AU - Abboussi, Oualid
N1 - Copyright © 2018. Published by Elsevier Ltd.
PY - 2019/2
Y1 - 2019/2
N2 - Chronic cannabinoid exposure during adolescence in male rats induces chronic cognitive and emotional impairments. However, the impact of this form of exposure on offspring vulnerability to stress is unknown. The aim of this study was to evaluate the behavioural and epigenetic effects of stress in the offspring of male rats whose fathers were exposed to cannabinoids during adolescence. Male adolescent offspring of Win55,212-2 (1.2 mg/kg) treated rats were exposed during one week to variable stressors and subjected to behavioural tests of anxiety and episodic-like memory, followed by an assessment of global DNA methylation and expression of DNA methyltransferases enzymes DNMT1 and DNMT3a mRNA in the prefrontal cortex. Stress exposure induced a significant anxiogenic-like effect but did not affect the episodic-like memory in the offspring of Win55,212-2 exposed fathers in comparison to the offspring of non-exposed fathers. These behavioural changes were subsequent to a significant increase in global DNA methylation and DNMT1 and DNMTa3 transcription in the prefrontal cortex. These data suggest that the deleterious effect of chronic exposure to cannabinoids during adolescence are not limited to the exposed individuals but may increase the vulnerability to stress-induced anxiety in the offspring and alter their epigenetic programming.
AB - Chronic cannabinoid exposure during adolescence in male rats induces chronic cognitive and emotional impairments. However, the impact of this form of exposure on offspring vulnerability to stress is unknown. The aim of this study was to evaluate the behavioural and epigenetic effects of stress in the offspring of male rats whose fathers were exposed to cannabinoids during adolescence. Male adolescent offspring of Win55,212-2 (1.2 mg/kg) treated rats were exposed during one week to variable stressors and subjected to behavioural tests of anxiety and episodic-like memory, followed by an assessment of global DNA methylation and expression of DNA methyltransferases enzymes DNMT1 and DNMT3a mRNA in the prefrontal cortex. Stress exposure induced a significant anxiogenic-like effect but did not affect the episodic-like memory in the offspring of Win55,212-2 exposed fathers in comparison to the offspring of non-exposed fathers. These behavioural changes were subsequent to a significant increase in global DNA methylation and DNMT1 and DNMTa3 transcription in the prefrontal cortex. These data suggest that the deleterious effect of chronic exposure to cannabinoids during adolescence are not limited to the exposed individuals but may increase the vulnerability to stress-induced anxiety in the offspring and alter their epigenetic programming.
KW - Adolescence
KW - Anxiety
KW - Cannabinoids
KW - DNA methylation
KW - Offspring
KW - Stress
UR - http://www.scopus.com/inward/record.url?scp=85057164395&partnerID=8YFLogxK
U2 - 10.1016/j.ijdevneu.2018.11.007
DO - 10.1016/j.ijdevneu.2018.11.007
M3 - Article
C2 - 30476535
VL - 72
SP - 48
EP - 54
JO - International Journal of Developmental Neuroscience
JF - International Journal of Developmental Neuroscience
SN - 0736-5748
ER -