Benzoyl peroxide increases UVA-induced plasma membrane damage and lipid oxidation in murine leukemia L1210 cells

Sally H. Ibbotson, Christopher R. Lambert, Michael N. Moran, Mary C. Lynch, Irene E. Kochevar

    Research output: Contribution to journalArticle

    11 Citations (Scopus)

    Abstract

    Ultraviolet A radiation induces oxidative stress and cell damage. The purpose of this investigation was to examine whether ultraviolet A-induced cell injury was amplified by the presence of a non-ultraviolet A absorbing molecule capable of generating free radicals. Benzoyl peroxide was used as a lipid soluble potential radical-generating agent. Plasma membrane permeability assessed by trypan blue uptake was used to measure cell damage in murine leukemia L1210 cells. Cells were irradiated with a pulsed Nd/YAG laser at 355 nm using 0-160 J per cm2. The ratio of the fluence-response slope in the presence of 40 microM benzoyl peroxide to that of irradiated controls was 4.3 +/- 2.6. Benzoyl peroxide alone or benzoyl peroxide added after irradiation did not cause increased trypan blue uptake. The ratio of the fluence-response slopes in the presence of 40 microM benzoyl peroxide to that of irradiated controls was 4.7 +/- 1.4 when cells were irradiated (0-43 J per cm2) with a xenon lamp, filtered to remove wavelengths 34 J per cm2. The increased trypan blue uptake and thiobarbituric acid reactive substances were inhibited by butylated hydroxytoluene. These results suggest that agents not absorbing ultraviolet A radiation may enhance ultraviolet A-initiated oxidative stress in cells.
    Original languageEnglish
    Pages (from-to)79-83
    Number of pages5
    JournalJournal of Investigative Dermatology
    Volume110
    Issue number1
    DOIs
    Publication statusPublished - Jan 1998

    Keywords

    • Animals
    • Ultraviolet Rays
    • Antioxidants
    • Thiobarbituric Acid Reactive Substances
    • Benzoyl Peroxide
    • Mice
    • Leukemia L1210
    • Oxidation-Reduction
    • Butylated Hydroxytoluene
    • Membrane Lipids
    • Oxidative Stress
    • Absorption
    • Cell Membrane
    • Cell Membrane Permeability
    • Lasers

    Fingerprint Dive into the research topics of 'Benzoyl peroxide increases UVA-induced plasma membrane damage and lipid oxidation in murine leukemia L1210 cells'. Together they form a unique fingerprint.

  • Cite this