Beta-papillomavirus DNA loads in hair follicles of immunocompetent people and organ transplant recipients

Soenke Weissenborn, Rachel E. Neale, Tim Waterboer, Damiano Abeni, Jan Nico Bouwes Bavinck, Adele C. Green, Catherine A. Harwood, Sylvie Euvrard, Mariet C. W. Feltkamp, Maurits N. C. de Koning, Luigi Naldi, Wim G. V. Quint, Gianpaolo Tessari, Charlotte M. Proby, Ulrike Wieland, Herbert Pfister

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    Abstract

    There is increasing evidence of an association between human papillomaviruses (HPV) of the beta-genus (beta-PV) and the development of cutaneous squamous cell carcinoma (SCC). The viral DNA load may be an important determinant of pathogenicity, but there are currently no baseline epidemiological data relating to load in people without SCC. We investigated DNA-loads of eight beta-PV types previously associated with risk of SCC. We collected eyebrow hairs from immunocompetent people (ICP) and organ transplant recipients (OTR), determined load by quantitative PCR and obtained demographic, phenotypic, and sun exposure information. Viral loads for ICP from Australia (n = 241) and Italy (n = 223) and OTR from across Europe (n = 318) spanned seven orders of magnitude. The median loads for all types were below one viral DNA copy per 60 cells and were highest for HPV5, HPV8 and HPV20. None of the populations had consistently higher viral loads for all 8 types. However, a higher proportion of OTR were in the top deciles of viral load distributions for six of the eight beta-PV types examined. In a nested analysis of Italian OTR and ICP, this finding was significant for six beta-PV types and cumulative load. Increasing age was significantly associated with higher viral loads in Australia, and there was a weak trend for higher loads with the time elapsed since transplantation in the OTR. We observed a wide distribution of beta-PV loads with OTR significantly more likely to have the highest viral loads. Thus, viral loads may be an important contributor to the higher risk of SCC in OTR.
    Original languageEnglish
    Pages (from-to)1-9
    Number of pages9
    JournalMedical Microbiology and Immunology
    Volume201
    Issue number2
    DOIs
    Publication statusPublished - 1 Jan 2012

    Fingerprint

    Hair Follicle
    Viral Load
    Transplants
    DNA
    Squamous Cell Carcinoma
    Viral DNA
    Eyebrows
    Solar System
    Transplant Recipients
    Hair
    Italy
    Virulence
    Transplantation
    Demography
    Polymerase Chain Reaction
    Skin
    Population

    Cite this

    Weissenborn, S., Neale, R. E., Waterboer, T., Abeni, D., Bavinck, J. N. B., Green, A. C., ... Pfister, H. (2012). Beta-papillomavirus DNA loads in hair follicles of immunocompetent people and organ transplant recipients. Medical Microbiology and Immunology, 201(2), 1-9. https://doi.org/10.1007/s00430-011-0212-3
    Weissenborn, Soenke ; Neale, Rachel E. ; Waterboer, Tim ; Abeni, Damiano ; Bavinck, Jan Nico Bouwes ; Green, Adele C. ; Harwood, Catherine A. ; Euvrard, Sylvie ; Feltkamp, Mariet C. W. ; de Koning, Maurits N. C. ; Naldi, Luigi ; Quint, Wim G. V. ; Tessari, Gianpaolo ; Proby, Charlotte M. ; Wieland, Ulrike ; Pfister, Herbert. / Beta-papillomavirus DNA loads in hair follicles of immunocompetent people and organ transplant recipients. In: Medical Microbiology and Immunology. 2012 ; Vol. 201, No. 2. pp. 1-9.
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    abstract = "There is increasing evidence of an association between human papillomaviruses (HPV) of the beta-genus (beta-PV) and the development of cutaneous squamous cell carcinoma (SCC). The viral DNA load may be an important determinant of pathogenicity, but there are currently no baseline epidemiological data relating to load in people without SCC. We investigated DNA-loads of eight beta-PV types previously associated with risk of SCC. We collected eyebrow hairs from immunocompetent people (ICP) and organ transplant recipients (OTR), determined load by quantitative PCR and obtained demographic, phenotypic, and sun exposure information. Viral loads for ICP from Australia (n = 241) and Italy (n = 223) and OTR from across Europe (n = 318) spanned seven orders of magnitude. The median loads for all types were below one viral DNA copy per 60 cells and were highest for HPV5, HPV8 and HPV20. None of the populations had consistently higher viral loads for all 8 types. However, a higher proportion of OTR were in the top deciles of viral load distributions for six of the eight beta-PV types examined. In a nested analysis of Italian OTR and ICP, this finding was significant for six beta-PV types and cumulative load. Increasing age was significantly associated with higher viral loads in Australia, and there was a weak trend for higher loads with the time elapsed since transplantation in the OTR. We observed a wide distribution of beta-PV loads with OTR significantly more likely to have the highest viral loads. Thus, viral loads may be an important contributor to the higher risk of SCC in OTR.",
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    Weissenborn, S, Neale, RE, Waterboer, T, Abeni, D, Bavinck, JNB, Green, AC, Harwood, CA, Euvrard, S, Feltkamp, MCW, de Koning, MNC, Naldi, L, Quint, WGV, Tessari, G, Proby, CM, Wieland, U & Pfister, H 2012, 'Beta-papillomavirus DNA loads in hair follicles of immunocompetent people and organ transplant recipients', Medical Microbiology and Immunology, vol. 201, no. 2, pp. 1-9. https://doi.org/10.1007/s00430-011-0212-3

    Beta-papillomavirus DNA loads in hair follicles of immunocompetent people and organ transplant recipients. / Weissenborn, Soenke; Neale, Rachel E.; Waterboer, Tim; Abeni, Damiano; Bavinck, Jan Nico Bouwes; Green, Adele C.; Harwood, Catherine A.; Euvrard, Sylvie; Feltkamp, Mariet C. W.; de Koning, Maurits N. C.; Naldi, Luigi; Quint, Wim G. V.; Tessari, Gianpaolo; Proby, Charlotte M.; Wieland, Ulrike; Pfister, Herbert.

    In: Medical Microbiology and Immunology, Vol. 201, No. 2, 01.01.2012, p. 1-9.

    Research output: Contribution to journalArticle

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    AU - Neale, Rachel E.

    AU - Waterboer, Tim

    AU - Abeni, Damiano

    AU - Bavinck, Jan Nico Bouwes

    AU - Green, Adele C.

    AU - Harwood, Catherine A.

    AU - Euvrard, Sylvie

    AU - Feltkamp, Mariet C. W.

    AU - de Koning, Maurits N. C.

    AU - Naldi, Luigi

    AU - Quint, Wim G. V.

    AU - Tessari, Gianpaolo

    AU - Proby, Charlotte M.

    AU - Wieland, Ulrike

    AU - Pfister, Herbert

    N1 - Copyright 2011 Elsevier B.V., All rights reserved.

    PY - 2012/1/1

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