Bimodal fluorogenic sensing of matrix proteolytic signatures in lung cancer

Alicia Megia-Fernandez, Bethany Mills, Chesney Michels, Sunay V. Chankeshwara, Nikola Krstajić, Chris Haslett, Kevin Dhaliwal, Mark Bradley

    Research output: Contribution to journalArticlepeer-review

    3 Citations (Scopus)
    134 Downloads (Pure)

    Abstract

    Optical biosensing based on the activation of fluorescent reporters offers a powerful methodology for the real-time molecular interrogation of pathology. Here we report a first-in-class, bimodal fluorescent reporter strategy for the simultaneous and highly specific detection of two independent proteases (thrombin and matrix metalloproteases (MMPs)) pivotal in the fibroproliferative process surrounding lung cancer, based on a dual, multiplexing, peptide FRET system. This sophisticated synthetic smartprobe, with a molecular weight of 6 kDa, contains two independent fluorophores and quenchers that generate photonic signatures at two specific wavelengths upon activation by target enzymes within human lung cancer tissue.

    Original languageEnglish
    Pages (from-to)8056-8063
    Number of pages8
    JournalOrganic and Biomolecular Chemistry
    Volume16
    Issue number43
    DOIs
    Publication statusPublished - 21 Nov 2018

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