Bimodal fluorogenic sensing of matrix proteolytic signatures in lung cancer

Alicia Megia-Fernandez; Bethany Mills; Chesney Michels; Sunay V. Chankeshwara; Nikola Krstajić; Chris Haslett; Kevin Dhaliwal; Mark Bradley

doi:10.1039/c8ob01790e

Bimodal fluorogenic sensing of matrix proteolytic signatures in lung cancer

Alicia Megia-Fernandez, Bethany Mills, Chesney Michels, Sunay V. Chankeshwara, Nikola Krstajić, Chris Haslett, Kevin Dhaliwal, Mark Bradley

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Abstract

Optical biosensing based on the activation of fluorescent reporters offers a powerful methodology for the real-time molecular interrogation of pathology. Here we report a first-in-class, bimodal fluorescent reporter strategy for the simultaneous and highly specific detection of two independent proteases (thrombin and matrix metalloproteases (MMPs)) pivotal in the fibroproliferative process surrounding lung cancer, based on a dual, multiplexing, peptide FRET system. This sophisticated synthetic smartprobe, with a molecular weight of 6 kDa, contains two independent fluorophores and quenchers that generate photonic signatures at two specific wavelengths upon activation by target enzymes within human lung cancer tissue.

Original language	English
Pages (from-to)	8056-8063
Number of pages	8
Journal	Organic and Biomolecular Chemistry
Volume	16
Issue number	43
DOIs	https://doi.org/10.1039/c8ob01790e
Publication status	Published - 21 Nov 2018

ASJC Scopus subject areas

Biochemistry
Physical and Theoretical Chemistry
Organic Chemistry

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10.1039/c8ob01790eLicence: CC BY

Final Published VersionFinal published version, 4.26 MBLicence: CC BY

Cite this

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T1 - Bimodal fluorogenic sensing of matrix proteolytic signatures in lung cancer

AU - Megia-Fernandez, Alicia

AU - Mills, Bethany

AU - Michels, Chesney

AU - Chankeshwara, Sunay V.

AU - Krstajić, Nikola

AU - Haslett, Chris

AU - Dhaliwal, Kevin

AU - Bradley, Mark

PY - 2018/11/21

Y1 - 2018/11/21

N2 - Optical biosensing based on the activation of fluorescent reporters offers a powerful methodology for the real-time molecular interrogation of pathology. Here we report a first-in-class, bimodal fluorescent reporter strategy for the simultaneous and highly specific detection of two independent proteases (thrombin and matrix metalloproteases (MMPs)) pivotal in the fibroproliferative process surrounding lung cancer, based on a dual, multiplexing, peptide FRET system. This sophisticated synthetic smartprobe, with a molecular weight of 6 kDa, contains two independent fluorophores and quenchers that generate photonic signatures at two specific wavelengths upon activation by target enzymes within human lung cancer tissue.

AB - Optical biosensing based on the activation of fluorescent reporters offers a powerful methodology for the real-time molecular interrogation of pathology. Here we report a first-in-class, bimodal fluorescent reporter strategy for the simultaneous and highly specific detection of two independent proteases (thrombin and matrix metalloproteases (MMPs)) pivotal in the fibroproliferative process surrounding lung cancer, based on a dual, multiplexing, peptide FRET system. This sophisticated synthetic smartprobe, with a molecular weight of 6 kDa, contains two independent fluorophores and quenchers that generate photonic signatures at two specific wavelengths upon activation by target enzymes within human lung cancer tissue.

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DO - 10.1039/c8ob01790e

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AN - SCOPUS:85056279451

SN - 1477-0520

VL - 16

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EP - 8063

JO - Organic and Biomolecular Chemistry

JF - Organic and Biomolecular Chemistry

IS - 43

ER -

Bimodal fluorogenic sensing of matrix proteolytic signatures in lung cancer

Abstract

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