Bimodal fluorogenic sensing of matrix proteolytic signatures in lung cancer

Alicia Megia-Fernandez, Bethany Mills, Chesney Michels, Sunay V. Chankeshwara, Nikola Krstajić, Chris Haslett, Kevin Dhaliwal, Mark Bradley

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    Abstract

    Optical biosensing based on the activation of fluorescent reporters offers a powerful methodology for the real-time molecular interrogation of pathology. Here we report a first-in-class, bimodal fluorescent reporter strategy for the simultaneous and highly specific detection of two independent proteases (thrombin and matrix metalloproteases (MMPs)) pivotal in the fibroproliferative process surrounding lung cancer, based on a dual, multiplexing, peptide FRET system. This sophisticated synthetic smartprobe, with a molecular weight of 6 kDa, contains two independent fluorophores and quenchers that generate photonic signatures at two specific wavelengths upon activation by target enzymes within human lung cancer tissue.

    Original languageEnglish
    Pages (from-to)8056-8063
    Number of pages8
    JournalOrganic and Biomolecular Chemistry
    Volume16
    Issue number43
    DOIs
    Publication statusPublished - 21 Nov 2018

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    Cite this

    Megia-Fernandez, A., Mills, B., Michels, C., Chankeshwara, S. V., Krstajić, N., Haslett, C., Dhaliwal, K., & Bradley, M. (2018). Bimodal fluorogenic sensing of matrix proteolytic signatures in lung cancer. Organic and Biomolecular Chemistry, 16(43), 8056-8063. https://doi.org/10.1039/c8ob01790e