TY - JOUR
T1 - Bioactive polymethoxylated flavonoids from Chiliadenus montanus
AU - Hamed, Ahmed R.
AU - Mohamed, Tarik A.
AU - Tawfik, Wafaa A.
AU - Hassan, Emad M.
AU - Higgins, Maureen
AU - El-Toumy, Sayed A.
AU - Dinkova-Kostova, Albena T.
N1 - This study was supported financially by Cancer Research UK (C20953/A18644) and National Research Centre in Egypt.
PY - 2016/3/31
Y1 - 2016/3/31
N2 - Phytochemical investigations of the CH2Cl2/MeOH (1:1) extract of air-dried aerial parts of Chiliadenus montanus afforded seven methoxylated flavonoids (1-7), three of them were isolated for the first time from the genus (1-3). Structures were established by spectroscopic methods, including HREIMS,1H,13C, DEPT,1H-1H COSY, HMQC and HMBC NMR analyses. In the present study, three of the isolated compounds were tested for the ability to induce NAD(P)H:quinoneoxidoreductase 1 (NQO1) using a quantitative bioassay in a murine hepatoma cell line. Compound 1 revealed dose-dependent NQO1 inducing properties with a concentration that doubled the specific enzyme activity by 2-fold (CD value) of 7.0 µM, and a magnitude of induction of 3.3-fold at the highest concentration tested (100 µM).
AB - Phytochemical investigations of the CH2Cl2/MeOH (1:1) extract of air-dried aerial parts of Chiliadenus montanus afforded seven methoxylated flavonoids (1-7), three of them were isolated for the first time from the genus (1-3). Structures were established by spectroscopic methods, including HREIMS,1H,13C, DEPT,1H-1H COSY, HMQC and HMBC NMR analyses. In the present study, three of the isolated compounds were tested for the ability to induce NAD(P)H:quinoneoxidoreductase 1 (NQO1) using a quantitative bioassay in a murine hepatoma cell line. Compound 1 revealed dose-dependent NQO1 inducing properties with a concentration that doubled the specific enzyme activity by 2-fold (CD value) of 7.0 µM, and a magnitude of induction of 3.3-fold at the highest concentration tested (100 µM).
UR - http://www.scopus.com/inward/record.url?scp=84979485181&partnerID=8YFLogxK
M3 - Article
VL - 8
SP - 788
EP - 793
JO - Journal of Chemical and Pharmaceutical Research
JF - Journal of Chemical and Pharmaceutical Research
IS - 3
ER -