Bioactive polymethoxylated flavonoids from Chiliadenus montanus

Ahmed R. Hamed; Tarik A. Mohamed; Wafaa A. Tawfik; Emad M. Hassan; Maureen Higgins; Sayed A. El-Toumy; Albena T. Dinkova-Kostova

Bioactive polymethoxylated flavonoids from Chiliadenus montanus

Ahmed R. Hamed (Lead / Corresponding author)
, Tarik A. Mohamed
, Wafaa A. Tawfik
, Emad M. Hassan
, Maureen Higgins
, Sayed A. El-Toumy
, Albena T. Dinkova-Kostova

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Abstract

Phytochemical investigations of the CH₂Cl₂/MeOH (1:1) extract of air-dried aerial parts of Chiliadenus montanus afforded seven methoxylated flavonoids (1-7), three of them were isolated for the first time from the genus (1-3). Structures were established by spectroscopic methods, including HREIMS,¹H,¹³C, DEPT,¹H-¹H COSY, HMQC and HMBC NMR analyses. In the present study, three of the isolated compounds were tested for the ability to induce NAD(P)H:quinoneoxidoreductase 1 (NQO1) using a quantitative bioassay in a murine hepatoma cell line. Compound 1 revealed dose-dependent NQO1 inducing properties with a concentration that doubled the specific enzyme activity by 2-fold (CD value) of 7.0 µM, and a magnitude of induction of 3.3-fold at the highest concentration tested (100 µM).

Original language	English
Pages (from-to)	788-793
Number of pages	6
Journal	Journal of Chemical and Pharmaceutical Research
Volume	8
Issue number	3
Publication status	Published - 31 Mar 2016

ASJC Scopus subject areas

Pharmaceutical Science

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http://jocpr.com/vol8-iss3.html

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@article{75a2cf0dc1b24231a4540116b38d6ef3,

title = "Bioactive polymethoxylated flavonoids from Chiliadenus montanus",

abstract = "Phytochemical investigations of the CH2Cl2/MeOH (1:1) extract of air-dried aerial parts of Chiliadenus montanus afforded seven methoxylated flavonoids (1-7), three of them were isolated for the first time from the genus (1-3). Structures were established by spectroscopic methods, including HREIMS,1H,13C, DEPT,1H-1H COSY, HMQC and HMBC NMR analyses. In the present study, three of the isolated compounds were tested for the ability to induce NAD(P)H:quinoneoxidoreductase 1 (NQO1) using a quantitative bioassay in a murine hepatoma cell line. Compound 1 revealed dose-dependent NQO1 inducing properties with a concentration that doubled the specific enzyme activity by 2-fold (CD value) of 7.0 µM, and a magnitude of induction of 3.3-fold at the highest concentration tested (100 µM).",

author = "Hamed, \{Ahmed R.\} and Mohamed, \{Tarik A.\} and Tawfik, \{Wafaa A.\} and Hassan, \{Emad M.\} and Maureen Higgins and El-Toumy, \{Sayed A.\} and Dinkova-Kostova, \{Albena T.\}",

note = "This study was supported financially by Cancer Research UK (C20953/A18644) and National Research Centre in Egypt. ",

year = "2016",

month = mar,

day = "31",

language = "English",

volume = "8",

pages = "788--793",

journal = "Journal of Chemical and Pharmaceutical Research",

publisher = "Journal of Chemical and Pharmaceutical Research",

number = "3",

}

TY - JOUR

T1 - Bioactive polymethoxylated flavonoids from Chiliadenus montanus

AU - Hamed, Ahmed R.

AU - Mohamed, Tarik A.

AU - Tawfik, Wafaa A.

AU - Hassan, Emad M.

AU - Higgins, Maureen

AU - El-Toumy, Sayed A.

AU - Dinkova-Kostova, Albena T.

N1 - This study was supported financially by Cancer Research UK (C20953/A18644) and National Research Centre in Egypt.

PY - 2016/3/31

Y1 - 2016/3/31

N2 - Phytochemical investigations of the CH2Cl2/MeOH (1:1) extract of air-dried aerial parts of Chiliadenus montanus afforded seven methoxylated flavonoids (1-7), three of them were isolated for the first time from the genus (1-3). Structures were established by spectroscopic methods, including HREIMS,1H,13C, DEPT,1H-1H COSY, HMQC and HMBC NMR analyses. In the present study, three of the isolated compounds were tested for the ability to induce NAD(P)H:quinoneoxidoreductase 1 (NQO1) using a quantitative bioassay in a murine hepatoma cell line. Compound 1 revealed dose-dependent NQO1 inducing properties with a concentration that doubled the specific enzyme activity by 2-fold (CD value) of 7.0 µM, and a magnitude of induction of 3.3-fold at the highest concentration tested (100 µM).

AB - Phytochemical investigations of the CH2Cl2/MeOH (1:1) extract of air-dried aerial parts of Chiliadenus montanus afforded seven methoxylated flavonoids (1-7), three of them were isolated for the first time from the genus (1-3). Structures were established by spectroscopic methods, including HREIMS,1H,13C, DEPT,1H-1H COSY, HMQC and HMBC NMR analyses. In the present study, three of the isolated compounds were tested for the ability to induce NAD(P)H:quinoneoxidoreductase 1 (NQO1) using a quantitative bioassay in a murine hepatoma cell line. Compound 1 revealed dose-dependent NQO1 inducing properties with a concentration that doubled the specific enzyme activity by 2-fold (CD value) of 7.0 µM, and a magnitude of induction of 3.3-fold at the highest concentration tested (100 µM).

UR - https://www.scopus.com/pages/publications/84979485181

M3 - Article

VL - 8

SP - 788

EP - 793

JO - Journal of Chemical and Pharmaceutical Research

JF - Journal of Chemical and Pharmaceutical Research

IS - 3

ER -

Bioactive polymethoxylated flavonoids from Chiliadenus montanus

Abstract

ASJC Scopus subject areas

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