Biochemical analysis of TssK, a core component of the bacterial Type VI secretion system, reveals distinct oligomeric states of TssK and identifies a TssK-TssFG sub-complex

Grant English, Olwyn Byron, Francesca R. Cianfanelli, Alan R. Prescott, Sarah J. Coulthurst (Lead / Corresponding author)

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    Abstract

    Gram-negative bacteria use the Type VI secretion system (T6SS) to inject toxic proteins into rival bacteria or eukaryotic cells. However the mechanism of the T6SS is incompletely understood. Here we studied a conserved component of the T6SS, TssK, using the anti-bacterial T6SS of Serratia marcescens as a model system. TssK was confirmed to be essential for effector secretion by the T6SS. The native protein, whilst not an integral membrane protein, appeared to localise to the inner membrane, consistent with its presence within a membrane-anchored assembly. Recombinant TssK purified from S. marcescens was found to exist in several stable oligomeric forms, namely trimer, hexamer and higher-order species. Native-level purification of TssK identified TssF and TssG as interacting proteins. TssF and TssG, conserved T6SS components of unknown function, were required for T6SS activity but not for correct localisation of TssK. A complex containing TssK, TssF and TssG was subsequently purified in vitro, confirming these three proteins form a new sub-complex within the T6SS. Our findings provide new insight into the T6SS assembly, allowing us to propose a model whereby TssK recruits TssFG into the membrane-associated T6SS complex and different oligomeric states of TssK may contribute to the dynamic mechanism of the system. © 2014 The Authors Journal compilation © 2014 Biochemical Society


    Original languageEnglish
    Pages (from-to)291-304
    Number of pages14
    JournalBiochemical Journal
    Volume461
    Issue number2
    DOIs
    Publication statusPublished - 2014

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    Serratia marcescens
    Membranes
    Bacteria
    Proteins
    Poisons
    Eukaryotic Cells
    Gram-Negative Bacteria
    Purification
    Membrane Proteins
    Type VI Secretion Systems
    In Vitro Techniques

    Cite this

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    title = "Biochemical analysis of TssK, a core component of the bacterial Type VI secretion system, reveals distinct oligomeric states of TssK and identifies a TssK-TssFG sub-complex",
    abstract = "Gram-negative bacteria use the Type VI secretion system (T6SS) to inject toxic proteins into rival bacteria or eukaryotic cells. However the mechanism of the T6SS is incompletely understood. Here we studied a conserved component of the T6SS, TssK, using the anti-bacterial T6SS of Serratia marcescens as a model system. TssK was confirmed to be essential for effector secretion by the T6SS. The native protein, whilst not an integral membrane protein, appeared to localise to the inner membrane, consistent with its presence within a membrane-anchored assembly. Recombinant TssK purified from S. marcescens was found to exist in several stable oligomeric forms, namely trimer, hexamer and higher-order species. Native-level purification of TssK identified TssF and TssG as interacting proteins. TssF and TssG, conserved T6SS components of unknown function, were required for T6SS activity but not for correct localisation of TssK. A complex containing TssK, TssF and TssG was subsequently purified in vitro, confirming these three proteins form a new sub-complex within the T6SS. Our findings provide new insight into the T6SS assembly, allowing us to propose a model whereby TssK recruits TssFG into the membrane-associated T6SS complex and different oligomeric states of TssK may contribute to the dynamic mechanism of the system. {\circledC} 2014 The Authors Journal compilation {\circledC} 2014 Biochemical Society normal{"}>",
    author = "Grant English and Olwyn Byron and Cianfanelli, {Francesca R.} and Prescott, {Alan R.} and Coulthurst, {Sarah J.}",
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    AU - English, Grant

    AU - Byron, Olwyn

    AU - Cianfanelli, Francesca R.

    AU - Prescott, Alan R.

    AU - Coulthurst, Sarah J.

    PY - 2014

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    N2 - Gram-negative bacteria use the Type VI secretion system (T6SS) to inject toxic proteins into rival bacteria or eukaryotic cells. However the mechanism of the T6SS is incompletely understood. Here we studied a conserved component of the T6SS, TssK, using the anti-bacterial T6SS of Serratia marcescens as a model system. TssK was confirmed to be essential for effector secretion by the T6SS. The native protein, whilst not an integral membrane protein, appeared to localise to the inner membrane, consistent with its presence within a membrane-anchored assembly. Recombinant TssK purified from S. marcescens was found to exist in several stable oligomeric forms, namely trimer, hexamer and higher-order species. Native-level purification of TssK identified TssF and TssG as interacting proteins. TssF and TssG, conserved T6SS components of unknown function, were required for T6SS activity but not for correct localisation of TssK. A complex containing TssK, TssF and TssG was subsequently purified in vitro, confirming these three proteins form a new sub-complex within the T6SS. Our findings provide new insight into the T6SS assembly, allowing us to propose a model whereby TssK recruits TssFG into the membrane-associated T6SS complex and different oligomeric states of TssK may contribute to the dynamic mechanism of the system. © 2014 The Authors Journal compilation © 2014 Biochemical Society normal">

    AB - Gram-negative bacteria use the Type VI secretion system (T6SS) to inject toxic proteins into rival bacteria or eukaryotic cells. However the mechanism of the T6SS is incompletely understood. Here we studied a conserved component of the T6SS, TssK, using the anti-bacterial T6SS of Serratia marcescens as a model system. TssK was confirmed to be essential for effector secretion by the T6SS. The native protein, whilst not an integral membrane protein, appeared to localise to the inner membrane, consistent with its presence within a membrane-anchored assembly. Recombinant TssK purified from S. marcescens was found to exist in several stable oligomeric forms, namely trimer, hexamer and higher-order species. Native-level purification of TssK identified TssF and TssG as interacting proteins. TssF and TssG, conserved T6SS components of unknown function, were required for T6SS activity but not for correct localisation of TssK. A complex containing TssK, TssF and TssG was subsequently purified in vitro, confirming these three proteins form a new sub-complex within the T6SS. Our findings provide new insight into the T6SS assembly, allowing us to propose a model whereby TssK recruits TssFG into the membrane-associated T6SS complex and different oligomeric states of TssK may contribute to the dynamic mechanism of the system. © 2014 The Authors Journal compilation © 2014 Biochemical Society normal">

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