Abstract
HLTF is highly similar in domain organisation to yeast Rad5. We identify PTIP and RPA70, both involved in DNA replication and DNA repair, as HLTF-interacting proteins although cells depleted of HLTF did not show defects in cellular responses to DNA damage. In vitro, HLTF has ATPase activity and E3 ubiquitin ligase activity with a range of E2 ubiquitin conjugating enzymes. HLTF expression is severely reduced in a range of cancer cells, and we suggest that the HLTF antibodies generated in this study could be used for cancer diagnostic purposes. (C) 2009 Elsevier Inc. All rights reserved.
Original language | English |
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Pages (from-to) | 187-191 |
Number of pages | 5 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 390 |
Issue number | 2 |
DOIs | |
Publication status | Published - 11 Dec 2009 |