Biochemical characterisation of the SWI/SNF family member HLTF

TY - JOUR

T1 - Biochemical characterisation of the SWI/SNF family member HLTF

AU - MacKay, Craig

AU - Toth, Rachel

AU - Rouse, John

PY - 2009/12/11

Y1 - 2009/12/11

N2 - HLTF is highly similar in domain organisation to yeast Rad5. We identify PTIP and RPA70, both involved in DNA replication and DNA repair, as HLTF-interacting proteins although cells depleted of HLTF did not show defects in cellular responses to DNA damage. In vitro, HLTF has ATPase activity and E3 ubiquitin ligase activity with a range of E2 ubiquitin conjugating enzymes. HLTF expression is severely reduced in a range of cancer cells, and we suggest that the HLTF antibodies generated in this study could be used for cancer diagnostic purposes. (C) 2009 Elsevier Inc. All rights reserved.

AB - HLTF is highly similar in domain organisation to yeast Rad5. We identify PTIP and RPA70, both involved in DNA replication and DNA repair, as HLTF-interacting proteins although cells depleted of HLTF did not show defects in cellular responses to DNA damage. In vitro, HLTF has ATPase activity and E3 ubiquitin ligase activity with a range of E2 ubiquitin conjugating enzymes. HLTF expression is severely reduced in a range of cancer cells, and we suggest that the HLTF antibodies generated in this study could be used for cancer diagnostic purposes. (C) 2009 Elsevier Inc. All rights reserved.

U2 - 10.1016/j.bbrc.2009.08.151

DO - 10.1016/j.bbrc.2009.08.151

M3 - Article

C2 - 19723507

SN - 0006-291X

VL - 390

SP - 187

EP - 191

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -