Bioengineered human tissue regeneration and repair using endogenous stem cells

Jiao Wei, Daniel T. Baptista-Hon, Zi Wang, Gen Li, Tanja Herrler, Chuanchang Dai, Kai Liu, Baofu Yu, Xiaoxue Chen, Mei Yang, Dong Han, Yuanxu Gao, Ru-Lin Huang, Lifei Guo (Lead / Corresponding author), Kang Zhang (Lead / Corresponding author), Qingfeng Li (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)
53 Downloads (Pure)

Abstract

We describe a general approach to produce bone and cartilaginous structures utilizing the self-regenerative capacity of the intercostal rib space to treat a deformed metacarpophalangeal joint and microtia. Anatomically precise 3D molds were positioned on the perichondro-periosteal or perichondral flap of the intercostal rib without any other exogenous elements. We find anatomically precise metacarpal head and auricle constructs within the implanted molds after 6 months. The regenerated metacarpal head was used successfully to surgically repair the deformed metacarpophalangeal joint. Auricle reconstructive surgery in five unilateral microtia patients yielded good aesthetic and functional results. Long-term follow-up revealed the auricle constructs were safe and stable. Single-cell RNA sequencing analysis reveal early infiltration of a cell population consistent with mesenchymal stem cells, followed by IL-8-stimulated differentiation into chondrocytes. Our results demonstrate the repair and regeneration of tissues using only endogenous factors and a viable treatment strategy for bone and tissue structural defects.

Original languageEnglish
Article number101156
Number of pages17
JournalCell Reports Medicine
Volume4
Issue number8
DOIs
Publication statusPublished - 15 Aug 2023

Keywords

  • auricular reconstruction
  • cartilage regeneration
  • endogenous stem cells
  • joint reconstruction
  • regeneration medicine
  • tissue regeneration

ASJC Scopus subject areas

  • General Biochemistry,Genetics and Molecular Biology

Fingerprint

Dive into the research topics of 'Bioengineered human tissue regeneration and repair using endogenous stem cells'. Together they form a unique fingerprint.

Cite this