TY - JOUR
T1 - Bioengineered human tissue regeneration and repair using endogenous stem cells
AU - Wei, Jiao
AU - Baptista-Hon, Daniel T.
AU - Wang, Zi
AU - Li, Gen
AU - Herrler, Tanja
AU - Dai, Chuanchang
AU - Liu, Kai
AU - Yu, Baofu
AU - Chen, Xiaoxue
AU - Yang, Mei
AU - Han, Dong
AU - Gao, Yuanxu
AU - Huang, Ru-Lin
AU - Guo, Lifei
AU - Zhang, Kang
AU - Li, Qingfeng
N1 - Funding Information:
This research was supported by grants from the Shanghai Municipal Key Clinical Specialty (grant no. shslczdzk00901), the National Natural Science Foundation of China (grant nos. 81871571, 81871572, 81702727), and Macau Science and Technology Development Fund, Macao (0007/2020/AFJ, 0070/2020/A2, 0109/2020/A3, 0003/2021/AKP). We would also like to thank Dr Hong Ouyang for expert discussions on single cell RNA sequencing results.
Copyright:
© 2023 The Authors. Published by Elsevier Inc. All rights reserved.
PY - 2023/8/15
Y1 - 2023/8/15
N2 - We describe a general approach to produce bone and cartilaginous structures utilizing the self-regenerative capacity of the intercostal rib space to treat a deformed metacarpophalangeal joint and microtia. Anatomically precise 3D molds were positioned on the perichondro-periosteal or perichondral flap of the intercostal rib without any other exogenous elements. We find anatomically precise metacarpal head and auricle constructs within the implanted molds after 6 months. The regenerated metacarpal head was used successfully to surgically repair the deformed metacarpophalangeal joint. Auricle reconstructive surgery in five unilateral microtia patients yielded good aesthetic and functional results. Long-term follow-up revealed the auricle constructs were safe and stable. Single-cell RNA sequencing analysis reveal early infiltration of a cell population consistent with mesenchymal stem cells, followed by IL-8-stimulated differentiation into chondrocytes. Our results demonstrate the repair and regeneration of tissues using only endogenous factors and a viable treatment strategy for bone and tissue structural defects.
AB - We describe a general approach to produce bone and cartilaginous structures utilizing the self-regenerative capacity of the intercostal rib space to treat a deformed metacarpophalangeal joint and microtia. Anatomically precise 3D molds were positioned on the perichondro-periosteal or perichondral flap of the intercostal rib without any other exogenous elements. We find anatomically precise metacarpal head and auricle constructs within the implanted molds after 6 months. The regenerated metacarpal head was used successfully to surgically repair the deformed metacarpophalangeal joint. Auricle reconstructive surgery in five unilateral microtia patients yielded good aesthetic and functional results. Long-term follow-up revealed the auricle constructs were safe and stable. Single-cell RNA sequencing analysis reveal early infiltration of a cell population consistent with mesenchymal stem cells, followed by IL-8-stimulated differentiation into chondrocytes. Our results demonstrate the repair and regeneration of tissues using only endogenous factors and a viable treatment strategy for bone and tissue structural defects.
KW - auricular reconstruction
KW - cartilage regeneration
KW - endogenous stem cells
KW - joint reconstruction
KW - regeneration medicine
KW - tissue regeneration
UR - http://www.scopus.com/inward/record.url?scp=85167799210&partnerID=8YFLogxK
U2 - 10.1016/j.xcrm.2023.101156
DO - 10.1016/j.xcrm.2023.101156
M3 - Article
C2 - 37586324
SN - 2666-3791
VL - 4
JO - Cell Reports Medicine
JF - Cell Reports Medicine
IS - 8
M1 - 101156
ER -