Bioengineered human tissue regeneration and repair using endogenous stem cells

Jiao Wei, Daniel T. Baptista-Hon, Zi Wang, Gen Li, Tanja Herrler, Chuanchang Dai, Kai Liu, Baofu Yu, Xiaoxue Chen, Mei Yang, Dong Han, Yuanxu Gao, Ru-Lin Huang, Lifei Guo (Lead / Corresponding author), Kang Zhang (Lead / Corresponding author), Qingfeng Li (Lead / Corresponding author)

    Research output: Contribution to journalArticlepeer-review

    16 Citations (Scopus)
    110 Downloads (Pure)

    Abstract

    We describe a general approach to produce bone and cartilaginous structures utilizing the self-regenerative capacity of the intercostal rib space to treat a deformed metacarpophalangeal joint and microtia. Anatomically precise 3D molds were positioned on the perichondro-periosteal or perichondral flap of the intercostal rib without any other exogenous elements. We find anatomically precise metacarpal head and auricle constructs within the implanted molds after 6 months. The regenerated metacarpal head was used successfully to surgically repair the deformed metacarpophalangeal joint. Auricle reconstructive surgery in five unilateral microtia patients yielded good aesthetic and functional results. Long-term follow-up revealed the auricle constructs were safe and stable. Single-cell RNA sequencing analysis reveal early infiltration of a cell population consistent with mesenchymal stem cells, followed by IL-8-stimulated differentiation into chondrocytes. Our results demonstrate the repair and regeneration of tissues using only endogenous factors and a viable treatment strategy for bone and tissue structural defects.

    Original languageEnglish
    Article number101156
    Number of pages17
    JournalCell Reports Medicine
    Volume4
    Issue number8
    DOIs
    Publication statusPublished - 15 Aug 2023

    Keywords

    • auricular reconstruction
    • cartilage regeneration
    • endogenous stem cells
    • joint reconstruction
    • regeneration medicine
    • tissue regeneration

    ASJC Scopus subject areas

    • General Biochemistry,Genetics and Molecular Biology

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