Bioengineered human tissue regeneration and repair using endogenous stem cells

TY - JOUR

T1 - Bioengineered human tissue regeneration and repair using endogenous stem cells

AU - Wei, Jiao

AU - Baptista-Hon, Daniel T.

AU - Wang, Zi

AU - Li, Gen

AU - Herrler, Tanja

AU - Dai, Chuanchang

AU - Liu, Kai

AU - Yu, Baofu

AU - Chen, Xiaoxue

AU - Yang, Mei

AU - Han, Dong

AU - Gao, Yuanxu

AU - Huang, Ru-Lin

AU - Guo, Lifei

AU - Zhang, Kang

AU - Li, Qingfeng

N1 - Funding Information: This research was supported by grants from the Shanghai Municipal Key Clinical Specialty (grant no. shslczdzk00901), the National Natural Science Foundation of China (grant nos. 81871571, 81871572, 81702727), and Macau Science and Technology Development Fund, Macao (0007/2020/AFJ, 0070/2020/A2, 0109/2020/A3, 0003/2021/AKP). We would also like to thank Dr Hong Ouyang for expert discussions on single cell RNA sequencing results. Copyright: © 2023 The Authors. Published by Elsevier Inc. All rights reserved.

PY - 2023/8/15

Y1 - 2023/8/15

N2 - We describe a general approach to produce bone and cartilaginous structures utilizing the self-regenerative capacity of the intercostal rib space to treat a deformed metacarpophalangeal joint and microtia. Anatomically precise 3D molds were positioned on the perichondro-periosteal or perichondral flap of the intercostal rib without any other exogenous elements. We find anatomically precise metacarpal head and auricle constructs within the implanted molds after 6 months. The regenerated metacarpal head was used successfully to surgically repair the deformed metacarpophalangeal joint. Auricle reconstructive surgery in five unilateral microtia patients yielded good aesthetic and functional results. Long-term follow-up revealed the auricle constructs were safe and stable. Single-cell RNA sequencing analysis reveal early infiltration of a cell population consistent with mesenchymal stem cells, followed by IL-8-stimulated differentiation into chondrocytes. Our results demonstrate the repair and regeneration of tissues using only endogenous factors and a viable treatment strategy for bone and tissue structural defects.

AB - We describe a general approach to produce bone and cartilaginous structures utilizing the self-regenerative capacity of the intercostal rib space to treat a deformed metacarpophalangeal joint and microtia. Anatomically precise 3D molds were positioned on the perichondro-periosteal or perichondral flap of the intercostal rib without any other exogenous elements. We find anatomically precise metacarpal head and auricle constructs within the implanted molds after 6 months. The regenerated metacarpal head was used successfully to surgically repair the deformed metacarpophalangeal joint. Auricle reconstructive surgery in five unilateral microtia patients yielded good aesthetic and functional results. Long-term follow-up revealed the auricle constructs were safe and stable. Single-cell RNA sequencing analysis reveal early infiltration of a cell population consistent with mesenchymal stem cells, followed by IL-8-stimulated differentiation into chondrocytes. Our results demonstrate the repair and regeneration of tissues using only endogenous factors and a viable treatment strategy for bone and tissue structural defects.

KW - auricular reconstruction

KW - cartilage regeneration

KW - endogenous stem cells

KW - joint reconstruction

KW - regeneration medicine

KW - tissue regeneration

UR - https://www.scopus.com/pages/publications/85167799210

U2 - 10.1016/j.xcrm.2023.101156

DO - 10.1016/j.xcrm.2023.101156

M3 - Article

C2 - 37586324

SN - 2666-3791

VL - 4

JO - Cell Reports Medicine

JF - Cell Reports Medicine

IS - 8

M1 - 101156

ER -