Bioinformatic prediction of putative conveyers of O-GlcNAc Transferase intellectual disability

Conor W. Mitchell, Ignacy Czajewski, Daan M. F. van Aalten (Lead / Corresponding author)

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)
    70 Downloads (Pure)

    Abstract

    Protein O-GlcNAcylation is a dynamic posttranslational modification that is catalyzed by the enzyme O-GlcNAc transferase (OGT) and is essential for neurodevelopment and postnatal neuronal function. Missense mutations in OGT segregate with a novel X-linked intellectual disability syndrome, the OGT congenital disorder of glycosylation (OGT-CDG). One hypothesis for the etiology of OGT-CDG is that loss of OGT activity leads to hypo-O-GlcNAcylation of as yet unidentified, specific neuronal proteins, affecting essential embryonic, and postnatal neurodevelopmental processes; however, the identity of these O-GlcNAcylated proteins is not known. Here, we used bioinformatic techniques to integrate sequence conservation, structural data, clinical data, and the available literature to identify 22 candidate proteins that convey OGT-CDG. We found using gene ontology and PANTHER database data that these candidate proteins are involved in diverse processes including Ras/MAPK signaling, translational repression, cytoskeletal dynamics, and chromatin remodeling. We also identify pathogenic missense variants at O-GlcNAcylation sites that segregate with intellectual disability. This work establishes a preliminary platform for the mechanistic dissection of the links between protein O-GlcNAcylation and neurodevelopment in OGT-CDG.

    Original languageEnglish
    Article number102276
    Pages (from-to)1-13
    Number of pages13
    JournalJournal of Biological Chemistry
    Volume298
    Issue number9
    Early online date19 Jul 2022
    DOIs
    Publication statusPublished - Sept 2022

    Keywords

    • O-GlcNAc
    • bioinformatics
    • cell signaling
    • gene expression
    • glycobiology
    • intellectual disability
    • neurodevelopment

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology
    • Cell Biology

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