Biomolecular layer thickness evaluation using White Light Reflectance Spectroscopy

M. Kitsara; P. Petrou; D. Kontziampasis; K. Misiakos; E. Makarona; I. Raptis; K. Beltsios

doi:10.1016/j.mee.2009.11.082

Biomolecular layer thickness evaluation using White Light Reflectance Spectroscopy

M. Kitsara (Lead / Corresponding author), P. Petrou, D. Kontziampasis, K. Misiakos, E. Makarona, I. Raptis, K. Beltsios

Mechanical and Industrial Engineering

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

A White Light Reflectance Spectroscopy (WLRS) methodology is applied in the evaluation of the effectiveness of biomolecules immobilization onto solid surfaces as well as their subsequent reaction with counterpart biomolecules via measuring the respective layers thickness. In particular we investigated the adsorption of rabbit and mouse gamma-globulins as well as their reaction with complementary antibodies. The results obtained with the proposed methodology were compared with those received by atomic force microscopy analysis of the same samples. It was found that the developed method provides a simple, very fast and accurate approach for thin biomolecular layers thickness determination.

Original language	English
Pages (from-to)	802-805
Number of pages	4
Journal	Microelectronic Engineering
Volume	87
Issue number	5-8
Early online date	22 Nov 2009
DOIs	https://doi.org/10.1016/j.mee.2009.11.082
Publication status	Published - May 2010

Keywords

Atomic force microscopy
Biomolecular layer
Gamma-globulins
Reflectance spectroscopy

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Condensed Matter Physics
Surfaces, Coatings and Films
Electrical and Electronic Engineering

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title = "Biomolecular layer thickness evaluation using White Light Reflectance Spectroscopy",

abstract = "A White Light Reflectance Spectroscopy (WLRS) methodology is applied in the evaluation of the effectiveness of biomolecules immobilization onto solid surfaces as well as their subsequent reaction with counterpart biomolecules via measuring the respective layers thickness. In particular we investigated the adsorption of rabbit and mouse gamma-globulins as well as their reaction with complementary antibodies. The results obtained with the proposed methodology were compared with those received by atomic force microscopy analysis of the same samples. It was found that the developed method provides a simple, very fast and accurate approach for thin biomolecular layers thickness determination.",

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AU - Kitsara, M.

AU - Petrou, P.

AU - Kontziampasis, D.

AU - Misiakos, K.

AU - Makarona, E.

AU - Raptis, I.

AU - Beltsios, K.

PY - 2010/5

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N2 - A White Light Reflectance Spectroscopy (WLRS) methodology is applied in the evaluation of the effectiveness of biomolecules immobilization onto solid surfaces as well as their subsequent reaction with counterpart biomolecules via measuring the respective layers thickness. In particular we investigated the adsorption of rabbit and mouse gamma-globulins as well as their reaction with complementary antibodies. The results obtained with the proposed methodology were compared with those received by atomic force microscopy analysis of the same samples. It was found that the developed method provides a simple, very fast and accurate approach for thin biomolecular layers thickness determination.

AB - A White Light Reflectance Spectroscopy (WLRS) methodology is applied in the evaluation of the effectiveness of biomolecules immobilization onto solid surfaces as well as their subsequent reaction with counterpart biomolecules via measuring the respective layers thickness. In particular we investigated the adsorption of rabbit and mouse gamma-globulins as well as their reaction with complementary antibodies. The results obtained with the proposed methodology were compared with those received by atomic force microscopy analysis of the same samples. It was found that the developed method provides a simple, very fast and accurate approach for thin biomolecular layers thickness determination.

KW - Atomic force microscopy

KW - Biomolecular layer

KW - Gamma-globulins

KW - Reflectance spectroscopy

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SN - 0167-9317

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EP - 805

JO - Microelectronic Engineering

JF - Microelectronic Engineering

IS - 5-8

ER -

Biomolecular layer thickness evaluation using White Light Reflectance Spectroscopy

Abstract

Keywords

ASJC Scopus subject areas

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