Blood monocytes and tumor-infiltrating macrophages in human breast cancer: differences in activation level as assessed by lysozyme content

Robert J. C. Steele, Oleg Eremin, Mary Brown

    Research output: Contribution to journalArticle

    7 Citations (Scopus)

    Abstract

    The lysozyme content of tumor-infiltrating macrophages (TIM) from human breast carcinomas has been compared with that of blood monocytes both from breast cancer patients and tumor-free controls. Cells were identified as macrophages or monocytes with the use of rosetting reactions to detect receptors for the Fc portion of IgG and differentiation antigens, and lysozyme was detected by an immunoperoxidase technique on cytocentrifuge preparations of rosetted cells. Significantly more monocytes from patients with breast cancer contained lysozyme than monocytes from comparable controls, suggesting the presence of activated circulating blood monocytes. Conversely, TIM were virtually devoid of lysozyme. This lack of enzyme was not due to methodologic factors and may represent defective antitumor activity.
    Original languageEnglish
    Pages (from-to)941-945
    Number of pages5
    JournalJournal of the National Cancer Institute
    Volume71
    Issue number5
    Publication statusPublished - 1983

    Fingerprint

    Muramidase
    Monocytes
    Macrophages
    Breast Neoplasms
    Neoplasms
    Fc Receptors
    Differentiation Antigens
    Immunoenzyme Techniques
    Immunoglobulin G
    Enzymes

    Keywords

    • Antigens, Surface
    • Breast Neoplasms
    • Cell Membrane
    • Female
    • Humans
    • Macrophage Activation
    • Macrophages
    • Microbial Collagenase
    • Monocytes
    • Muramidase
    • Receptors, Fc
    • Rosette Formation

    Cite this

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    title = "Blood monocytes and tumor-infiltrating macrophages in human breast cancer: differences in activation level as assessed by lysozyme content",
    abstract = "The lysozyme content of tumor-infiltrating macrophages (TIM) from human breast carcinomas has been compared with that of blood monocytes both from breast cancer patients and tumor-free controls. Cells were identified as macrophages or monocytes with the use of rosetting reactions to detect receptors for the Fc portion of IgG and differentiation antigens, and lysozyme was detected by an immunoperoxidase technique on cytocentrifuge preparations of rosetted cells. Significantly more monocytes from patients with breast cancer contained lysozyme than monocytes from comparable controls, suggesting the presence of activated circulating blood monocytes. Conversely, TIM were virtually devoid of lysozyme. This lack of enzyme was not due to methodologic factors and may represent defective antitumor activity.",
    keywords = "Antigens, Surface, Breast Neoplasms, Cell Membrane, Female, Humans, Macrophage Activation, Macrophages, Microbial Collagenase, Monocytes, Muramidase, Receptors, Fc, Rosette Formation",
    author = "Steele, {Robert J. C.} and Oleg Eremin and Mary Brown",
    year = "1983",
    language = "English",
    volume = "71",
    pages = "941--945",
    journal = "Journal of the National Cancer Institute",
    issn = "0027-8874",
    publisher = "Oxford University Press",
    number = "5",

    }

    TY - JOUR

    T1 - Blood monocytes and tumor-infiltrating macrophages in human breast cancer

    T2 - differences in activation level as assessed by lysozyme content

    AU - Steele, Robert J. C.

    AU - Eremin, Oleg

    AU - Brown, Mary

    PY - 1983

    Y1 - 1983

    N2 - The lysozyme content of tumor-infiltrating macrophages (TIM) from human breast carcinomas has been compared with that of blood monocytes both from breast cancer patients and tumor-free controls. Cells were identified as macrophages or monocytes with the use of rosetting reactions to detect receptors for the Fc portion of IgG and differentiation antigens, and lysozyme was detected by an immunoperoxidase technique on cytocentrifuge preparations of rosetted cells. Significantly more monocytes from patients with breast cancer contained lysozyme than monocytes from comparable controls, suggesting the presence of activated circulating blood monocytes. Conversely, TIM were virtually devoid of lysozyme. This lack of enzyme was not due to methodologic factors and may represent defective antitumor activity.

    AB - The lysozyme content of tumor-infiltrating macrophages (TIM) from human breast carcinomas has been compared with that of blood monocytes both from breast cancer patients and tumor-free controls. Cells were identified as macrophages or monocytes with the use of rosetting reactions to detect receptors for the Fc portion of IgG and differentiation antigens, and lysozyme was detected by an immunoperoxidase technique on cytocentrifuge preparations of rosetted cells. Significantly more monocytes from patients with breast cancer contained lysozyme than monocytes from comparable controls, suggesting the presence of activated circulating blood monocytes. Conversely, TIM were virtually devoid of lysozyme. This lack of enzyme was not due to methodologic factors and may represent defective antitumor activity.

    KW - Antigens, Surface

    KW - Breast Neoplasms

    KW - Cell Membrane

    KW - Female

    KW - Humans

    KW - Macrophage Activation

    KW - Macrophages

    KW - Microbial Collagenase

    KW - Monocytes

    KW - Muramidase

    KW - Receptors, Fc

    KW - Rosette Formation

    M3 - Article

    C2 - 6316013

    VL - 71

    SP - 941

    EP - 945

    JO - Journal of the National Cancer Institute

    JF - Journal of the National Cancer Institute

    SN - 0027-8874

    IS - 5

    ER -