Branch migration during homologous recombination: assembly of a RuvAB-Holliday junction complex in vitro

Kevin Hiom, Stephen C. West

    Research output: Contribution to journalArticle

    57 Citations (Scopus)

    Abstract

    The RuvA and RuvB proteins of E. coli promote the branch migration or movement of Holliday junctions during genetic recombination and DNA repair. Using small synthetic Holliday junctions in which the crossover point is confined near one end of the DNA molecule, we show that RuvAB-mediated branch migration occurs with a defined polarity. The assembly of RuvA and RuvB on the Holliday junction has been investigated by sedimentation analysis and by DNase I footprinting. We find that RuvA protein binds and protects all four strands of DNA at the crossover point, whereas RuvB protein binds the DNA asymmetrically. The polarity of branch migration is defined by the asymmetric assembly of the RuvAB branch migration complex relative to the junction and is consistent with a model in which RuvAB drives branch migration by passing the DNA through the hexameric rings of RuvB.

    Original languageEnglish
    Pages (from-to)787-793
    Number of pages7
    JournalCell
    Volume80
    Issue number5
    DOIs
    Publication statusPublished - 10 Mar 1995

    Fingerprint

    Cruciform DNA
    Homologous Recombination
    DNA
    Recombinational DNA Repair
    Deoxyribonuclease I
    DNA Repair
    Genetic Recombination
    Escherichia coli Proteins
    Proteins
    Sedimentation
    Repair
    In Vitro Techniques
    Molecules

    Keywords

    • Adenosine triphosphate
    • Bacterial proteins
    • Base sequence
    • DNA helicases
    • DNA repair
    • DNA, Bacterial
    • DNA-binding proteins
    • Escherichia coli
    • Escherichia coli proteins
    • Models, Genetic
    • Models, Molecular
    • Molecular sequence data
    • Recombination, Genetic

    Cite this

    @article{4bce30abae164a959ed0ac02a4c55b4f,
    title = "Branch migration during homologous recombination: assembly of a RuvAB-Holliday junction complex in vitro",
    abstract = "The RuvA and RuvB proteins of E. coli promote the branch migration or movement of Holliday junctions during genetic recombination and DNA repair. Using small synthetic Holliday junctions in which the crossover point is confined near one end of the DNA molecule, we show that RuvAB-mediated branch migration occurs with a defined polarity. The assembly of RuvA and RuvB on the Holliday junction has been investigated by sedimentation analysis and by DNase I footprinting. We find that RuvA protein binds and protects all four strands of DNA at the crossover point, whereas RuvB protein binds the DNA asymmetrically. The polarity of branch migration is defined by the asymmetric assembly of the RuvAB branch migration complex relative to the junction and is consistent with a model in which RuvAB drives branch migration by passing the DNA through the hexameric rings of RuvB.",
    keywords = "Adenosine triphosphate, Bacterial proteins, Base sequence, DNA helicases, DNA repair, DNA, Bacterial, DNA-binding proteins, Escherichia coli, Escherichia coli proteins, Models, Genetic, Models, Molecular, Molecular sequence data, Recombination, Genetic",
    author = "Kevin Hiom and West, {Stephen C.}",
    year = "1995",
    month = "3",
    day = "10",
    doi = "10.1016/0092-8674(95)90357-7",
    language = "English",
    volume = "80",
    pages = "787--793",
    journal = "Cell",
    issn = "0092-8674",
    publisher = "Elsevier",
    number = "5",

    }

    Branch migration during homologous recombination : assembly of a RuvAB-Holliday junction complex in vitro. / Hiom, Kevin; West, Stephen C.

    In: Cell, Vol. 80, No. 5, 10.03.1995, p. 787-793.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Branch migration during homologous recombination

    T2 - assembly of a RuvAB-Holliday junction complex in vitro

    AU - Hiom, Kevin

    AU - West, Stephen C.

    PY - 1995/3/10

    Y1 - 1995/3/10

    N2 - The RuvA and RuvB proteins of E. coli promote the branch migration or movement of Holliday junctions during genetic recombination and DNA repair. Using small synthetic Holliday junctions in which the crossover point is confined near one end of the DNA molecule, we show that RuvAB-mediated branch migration occurs with a defined polarity. The assembly of RuvA and RuvB on the Holliday junction has been investigated by sedimentation analysis and by DNase I footprinting. We find that RuvA protein binds and protects all four strands of DNA at the crossover point, whereas RuvB protein binds the DNA asymmetrically. The polarity of branch migration is defined by the asymmetric assembly of the RuvAB branch migration complex relative to the junction and is consistent with a model in which RuvAB drives branch migration by passing the DNA through the hexameric rings of RuvB.

    AB - The RuvA and RuvB proteins of E. coli promote the branch migration or movement of Holliday junctions during genetic recombination and DNA repair. Using small synthetic Holliday junctions in which the crossover point is confined near one end of the DNA molecule, we show that RuvAB-mediated branch migration occurs with a defined polarity. The assembly of RuvA and RuvB on the Holliday junction has been investigated by sedimentation analysis and by DNase I footprinting. We find that RuvA protein binds and protects all four strands of DNA at the crossover point, whereas RuvB protein binds the DNA asymmetrically. The polarity of branch migration is defined by the asymmetric assembly of the RuvAB branch migration complex relative to the junction and is consistent with a model in which RuvAB drives branch migration by passing the DNA through the hexameric rings of RuvB.

    KW - Adenosine triphosphate

    KW - Bacterial proteins

    KW - Base sequence

    KW - DNA helicases

    KW - DNA repair

    KW - DNA, Bacterial

    KW - DNA-binding proteins

    KW - Escherichia coli

    KW - Escherichia coli proteins

    KW - Models, Genetic

    KW - Models, Molecular

    KW - Molecular sequence data

    KW - Recombination, Genetic

    U2 - 10.1016/0092-8674(95)90357-7

    DO - 10.1016/0092-8674(95)90357-7

    M3 - Article

    C2 - 7889572

    VL - 80

    SP - 787

    EP - 793

    JO - Cell

    JF - Cell

    SN - 0092-8674

    IS - 5

    ER -