BTB-BACK Domain Protein POB1 Suppresses Immune Cell Death by Targeting Ubiquitin E3 ligase PUB17 for Degradation

Beatriz Orosa, Qin He, Joelle Mesmar, Eleanor M. Gilroy, Hazel McLellan, Chengwei Yang, Adam Craig, Mark Bailey, Cunjin Zhang, Jonathan David Moore, Petra C. Boevink, Zhendong Tian, Paul R. J. Birch (Lead / Corresponding author), Ari Sadanandom (Lead / Corresponding author)

Research output: Contribution to journalArticle

16 Citations (Scopus)
211 Downloads (Pure)

Abstract

Hypersensitive response programmed cell death (HR-PCD) is a critical feature in plant immunity required for pathogen restriction and prevention of disease development. The precise control of this process is paramount to cell survival and an effective immune response. The discovery of new components that function to suppress HR-PCD will be instrumental in understanding the regulation of this fundamental mechanism. Here we report the identification and characterisation of a BTB domain E3 ligase protein, POB1, that functions to suppress HR-PCD triggered by evolutionarily diverse pathogens. Nicotiana benthamiana and tobacco plants with reduced POB1 activity show accelerated HR-PCD whilst those with increased POB1 levels show attenuated HR-PCD. We demonstrate that POB1 dimerization and nuclear localization are vital for its function in HR-PCD suppression. Using protein-protein interaction assays, we identify the Plant U-Box E3 ligase PUB17, a well established positive regulator of plant innate immunity, as a target for POB1-mediated proteasomal degradation. Using confocal imaging and in planta immunoprecipitation assays we show that POB1 interacts with PUB17 in the nucleus and stimulates its degradation. Mutated versions of POB1 that show reduced interaction with PUB17 fail to suppress HR-PCD, indicating that POB1-mediated degradation of PUB17 U-box E3 ligase is an important step for negative regulation of specific immune pathways in plants. Our data reveals a new mechanism for BTB domain proteins in suppressing HR-PCD in plant innate immune responses.

Original languageEnglish
Article numbere1006540
Pages (from-to)1-26
Number of pages26
JournalPLoS Genetics
Volume13
Issue number1
DOIs
Publication statusPublished - 5 Jan 2017

Fingerprint Dive into the research topics of 'BTB-BACK Domain Protein POB1 Suppresses Immune Cell Death by Targeting Ubiquitin E3 ligase PUB17 for Degradation'. Together they form a unique fingerprint.

  • Projects

    Profiles

    No photo of Paul Birch

    Birch, Paul

    Person: Academic

    Cite this

    Orosa, B., He, Q., Mesmar, J., Gilroy, E. M., McLellan, H., Yang, C., Craig, A., Bailey, M., Zhang, C., Moore, J. D., Boevink, P. C., Tian, Z., Birch, P. R. J., & Sadanandom, A. (2017). BTB-BACK Domain Protein POB1 Suppresses Immune Cell Death by Targeting Ubiquitin E3 ligase PUB17 for Degradation. PLoS Genetics, 13(1), 1-26. [e1006540]. https://doi.org/10.1371/journal.pgen.1006540