Burkholderia phymatum is a highly effective nitrogen-fixing symbiont of Mimosa spp. and fixes nitrogen ex planta

Geoffrey N. Elliott, Wen-Ming Chen, Jui-Hsing Chou, Hui-Chun Wang, Shih-Yi Sheu, Liamara Perin, Veronica M Reis, Lionel Moulin, Marcelo F. Simon, Cyril Bontemps, Joan M. Sutherland, Rosana Bessi, Sergio M de Faria, Michael J Trinick, Alan R. Prescott, Janet I. Sprent, Euan K. James

    Research output: Contribution to journalArticlepeer-review

    139 Citations (Scopus)

    Abstract

    * The ability of Burkholderia phymatum STM815 to effectively nodulate Mimosa spp., and to fix nitrogen ex planta, was compared with that of the known Mimosa symbiont Cupriavidus taiwanensis LMG19424. * Both strains were equally effective symbionts of M. pudica, but nodules formed by STM815 had greater nitrogenase activity. STM815 was shown to have a broader host range across the genus Mimosa than LMG19424, nodulating 30 out of 31 species, 21 of these effectively. LMG19424 effectively nodulated only nine species. GFP-marked variants were used to visualise symbiont presence within nodules. * STM815 gave significant acetylene reduction assay (ARA) activity in semisolid JMV medium ex planta, but no ARA activity was detected with LMG19424. 16S rDNA sequences of two isolates originally from Mimosa nodules in Papua New Guinea (NGR114 and NGR195A) identified them as Burkholderia phymatum also, with nodA, nodC and nifH genes of NGR195A identical to those of STM815. * B. phymatum is therefore an effective Mimosa symbiont with a broad host range, and is the first reported beta-rhizobial strain to fix nitrogen in free-living culture.
    Original languageEnglish
    Pages (from-to)168-180
    Number of pages13
    JournalNew Phytologist
    Volume173
    Issue number1
    DOIs
    Publication statusPublished - 2007

    Keywords

    • Bacterial Proteins
    • Bacteriological Techniques
    • Burkholderia
    • Cupriavidus
    • DNA, Ribosomal
    • Fabaceae
    • Green Fluorescent Proteins
    • Mimosa
    • Nitrogenase
    • Phylogeny
    • Root Nodules, Plant
    • Species Specificity
    • Symbiosis

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