Burkholderia symbiotica sp. nov., isolated from root nodules of mimosa spp. native to north-east Brazil

Four strains, designated JPY-345 , JPY-347, JPY-366 and JPY-581, were isolated from nitrogenfixing nodules on the roots of two species of Mimosa, Mimosa cordistipula and Mimosa misera, that are native to North East Brazil, and their taxonomic positions were investigated by using a polyphasic approach. All four strains grew at 15-43°C (optimum 35°C), at pH 4-7 (optimum pH 5) and with 0-2% (w/v) NaCl (optimum 0% NaCl). On the basis of 16S rRNA gene sequence analysis, strain JPY-345 showed 97.3% sequence similarity to the closest relatedspecies Burkholderia soli GP25-8 , 97.3% sequence similarity to Burkholderia caryophylli ATCC25418 and 97.1% sequence similarity to Burkholderia kururiensis KP23 . The predominant fatty acids of the strains were C ?7c (36.1 %), C (19.8 %) and summed feature 3, comprising C ?7c and/or C ?6c (11.5 %). The major isoprenoid quinone was Q-8 and the DNA G+C content of the strains was 64.2-65.7 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and several uncharacterized aminophospholipids and phospholipids. DNA-DNA hybridizations between the novel strain and recognized species of the genus Burkholderia yielded relatedness values of