BV-2 microglial cells sense micro-nanotextured silicon surface topology

Saydulla Persheyev, Yongchang Fan, Andrew Irving, Mervyn J. Rose

    Research output: Contribution to journalArticlepeer-review

    9 Citations (Scopus)

    Abstract

    Artificial biomimetic substrates provide useful models for studying cell adhesion, signaling, and differentiation. This article describes biological interactions with a new type of tunable, micro-nanotextured silicon substrate, generated by irradiation of a hydrogenated amorphous silicon film with a large beam, excimer laser (248 nm). In this study, we demonstrate that BV-2 microglial cells can sense differences in laser processed silicon surface topology over the range of 30 nm to 2 mu m, where they undergo marked morphogenic changes with increasing feature size. The cells adopt a more elongated shape in the presence of the modified surface structure and exhibit increased levels of actin-rich microdomains, suggesting enhanced adhesion. The excimer laser modification of hydrogenated amorphous silicon to generate micro-nanostructures realizes large area benefits as well as providing a biomaterial where the external and internal structure can be altered and tuned for various applications. (C) 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 99A: 135-140, 2011.

    Original languageEnglish
    Pages (from-to)135-140
    Number of pages6
    JournalJournal of Biomedical Materials Research Part A
    Volume99A
    Issue number1
    DOIs
    Publication statusPublished - Oct 2011

    Keywords

    • cell adhesion
    • cell signaling and interactions
    • BV-2 microglia cells
    • micro-nanotextured silicon
    • cytoskeleton
    • actin
    • OSTEOPROGENITOR RESPONSE
    • STEM-CELLS
    • EXCIMER
    • NANOTOPOGRAPHY
    • DYNAMICS
    • CNS

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