BVT.3531 reduces body weight and activates KATP channels in isolated arcuate neurons in rats

Shirin Mirshamsi, Madelene Olsson, Urban Arnelo, Jackie M. Kinsella, Johan Permert, Michael L. J. Ashford

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    A number of hormones, including leptin, have been shown to inhibit food intake in humans and animals. Analogues of 3-guanidinopropionic acid have also been found to reduce total food intake, but their mechanisms of action have not been well studied. The present study investigated the effects of intracerebroventricular infusion of the analogue BVT.3531 on food intake, meal pattern, and body weight in rats during 7 days. Single channel recordings from arcuate neurons and insulinoma cells were used to determine the effects of BVT.3531 on K(ATP) activity. Data analysis showed that BVT.3531 significantly decreased body weight and food intake, primarily by reducing meal size. BVT.3531 activated KATP channels in cell-attached recordings from insulin-secreting cells and rat arcuate neurons but had no effect on KATP channel activity in inside-out membrane patches from either cell type. BVT.3531 did not alter the firing rate or K+ channel activity of arcuate neurons devoid of KATP. The study suggests that small molecules capable of mimicking the effects of leptin on food intake and body weight may utilize output mechanisms similar to those of leptin to elicit changes in arcuate neuron excitability.

    Original languageEnglish
    Pages (from-to)19-24
    Number of pages6
    JournalRegulatory Peptides
    Volume141
    Issue number1-3
    DOIs
    Publication statusPublished - Jun 2007

    Fingerprint

    KATP Channels
    Neurons
    Rats
    Eating
    Body Weight
    Leptin
    Meals
    Intraventricular Infusions
    Insulinoma
    Insulin-Secreting Cells
    Animals
    Adenosine Triphosphate
    2-(1-(hydrazino(imino)methyl)hydrazino)acetic acid
    Hormones
    Insulin
    Membranes
    Molecules

    Cite this

    Mirshamsi, Shirin ; Olsson, Madelene ; Arnelo, Urban ; Kinsella, Jackie M. ; Permert, Johan ; Ashford, Michael L. J. / BVT.3531 reduces body weight and activates KATP channels in isolated arcuate neurons in rats. In: Regulatory Peptides. 2007 ; Vol. 141, No. 1-3. pp. 19-24.
    @article{d059498fb1b24804a92074e50d95435f,
    title = "BVT.3531 reduces body weight and activates KATP channels in isolated arcuate neurons in rats",
    abstract = "A number of hormones, including leptin, have been shown to inhibit food intake in humans and animals. Analogues of 3-guanidinopropionic acid have also been found to reduce total food intake, but their mechanisms of action have not been well studied. The present study investigated the effects of intracerebroventricular infusion of the analogue BVT.3531 on food intake, meal pattern, and body weight in rats during 7 days. Single channel recordings from arcuate neurons and insulinoma cells were used to determine the effects of BVT.3531 on K(ATP) activity. Data analysis showed that BVT.3531 significantly decreased body weight and food intake, primarily by reducing meal size. BVT.3531 activated KATP channels in cell-attached recordings from insulin-secreting cells and rat arcuate neurons but had no effect on KATP channel activity in inside-out membrane patches from either cell type. BVT.3531 did not alter the firing rate or K+ channel activity of arcuate neurons devoid of KATP. The study suggests that small molecules capable of mimicking the effects of leptin on food intake and body weight may utilize output mechanisms similar to those of leptin to elicit changes in arcuate neuron excitability.",
    author = "Shirin Mirshamsi and Madelene Olsson and Urban Arnelo and Kinsella, {Jackie M.} and Johan Permert and Ashford, {Michael L. J.}",
    year = "2007",
    month = "6",
    doi = "10.1016/j.regpep.2006.12.009",
    language = "English",
    volume = "141",
    pages = "19--24",
    journal = "Regulatory Peptides",
    issn = "0167-0115",
    publisher = "Elsevier",
    number = "1-3",

    }

    BVT.3531 reduces body weight and activates KATP channels in isolated arcuate neurons in rats. / Mirshamsi, Shirin; Olsson, Madelene; Arnelo, Urban; Kinsella, Jackie M.; Permert, Johan; Ashford, Michael L. J.

    In: Regulatory Peptides, Vol. 141, No. 1-3, 06.2007, p. 19-24.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - BVT.3531 reduces body weight and activates KATP channels in isolated arcuate neurons in rats

    AU - Mirshamsi, Shirin

    AU - Olsson, Madelene

    AU - Arnelo, Urban

    AU - Kinsella, Jackie M.

    AU - Permert, Johan

    AU - Ashford, Michael L. J.

    PY - 2007/6

    Y1 - 2007/6

    N2 - A number of hormones, including leptin, have been shown to inhibit food intake in humans and animals. Analogues of 3-guanidinopropionic acid have also been found to reduce total food intake, but their mechanisms of action have not been well studied. The present study investigated the effects of intracerebroventricular infusion of the analogue BVT.3531 on food intake, meal pattern, and body weight in rats during 7 days. Single channel recordings from arcuate neurons and insulinoma cells were used to determine the effects of BVT.3531 on K(ATP) activity. Data analysis showed that BVT.3531 significantly decreased body weight and food intake, primarily by reducing meal size. BVT.3531 activated KATP channels in cell-attached recordings from insulin-secreting cells and rat arcuate neurons but had no effect on KATP channel activity in inside-out membrane patches from either cell type. BVT.3531 did not alter the firing rate or K+ channel activity of arcuate neurons devoid of KATP. The study suggests that small molecules capable of mimicking the effects of leptin on food intake and body weight may utilize output mechanisms similar to those of leptin to elicit changes in arcuate neuron excitability.

    AB - A number of hormones, including leptin, have been shown to inhibit food intake in humans and animals. Analogues of 3-guanidinopropionic acid have also been found to reduce total food intake, but their mechanisms of action have not been well studied. The present study investigated the effects of intracerebroventricular infusion of the analogue BVT.3531 on food intake, meal pattern, and body weight in rats during 7 days. Single channel recordings from arcuate neurons and insulinoma cells were used to determine the effects of BVT.3531 on K(ATP) activity. Data analysis showed that BVT.3531 significantly decreased body weight and food intake, primarily by reducing meal size. BVT.3531 activated KATP channels in cell-attached recordings from insulin-secreting cells and rat arcuate neurons but had no effect on KATP channel activity in inside-out membrane patches from either cell type. BVT.3531 did not alter the firing rate or K+ channel activity of arcuate neurons devoid of KATP. The study suggests that small molecules capable of mimicking the effects of leptin on food intake and body weight may utilize output mechanisms similar to those of leptin to elicit changes in arcuate neuron excitability.

    U2 - 10.1016/j.regpep.2006.12.009

    DO - 10.1016/j.regpep.2006.12.009

    M3 - Article

    VL - 141

    SP - 19

    EP - 24

    JO - Regulatory Peptides

    JF - Regulatory Peptides

    SN - 0167-0115

    IS - 1-3

    ER -