c-Myc co-ordinates mRNA cap methylation and ribosomal RNA production

Sianadh Dunn; Olivia Lombardi; Victoria Cowling

doi:10.1042/BCJ20160930

c-Myc co-ordinates mRNA cap methylation and ribosomal RNA production

Sianadh Dunn, Olivia Lombardi, Victoria Cowling

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

201 Downloads (Pure)

Abstract

The mRNA cap is a structure added to RNA pol II transcripts in eukaryotes, which recruits factors involved in RNA processing, nuclear export and translation initiation. RNMT-RAM, the mRNA cap methyltransferase complex, completes the basic functional mRNA cap structure, cap 0, by methylating the cap guanosine. Here we report that RNMT-RAM co-ordinates mRNA processing with ribosome production. Suppression of RNMT-RAM reduces synthesis of the 45S ribosomal RNA (rRNA) precursor. RNMT- RAM is required for c-Myc expression, a major regulator of RNA pol I, which synthesises 45S rRNA. Constitutive expression of c-Myc restores rRNA synthesis when RNMT-RAM is suppressed, indicating that RNMT-RAM controls rRNA production predominantly by controlling c-Myc expression. We report that RNMT-RAM is recruited to the rDNA locus, which may contribute to rRNA synthesis in certain contexts.

Original language	English
Pages (from-to)	377-384
Number of pages	8
Journal	Biochemical Journal
Volume	474
Issue number	3
Early online date	1 Dec 2016
DOIs	https://doi.org/10.1042/BCJ20160930
Publication status	Published - 20 Jan 2017

Keywords

RNMT-RAM
c-Myc
rRNA
transcription
mRNA cap
mRNA

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10.1042/BCJ20160930Licence: CC BY

Final published version
© 2017 The Author(s). This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY).
Final published version, 566 KBLicence: CC BY

Investigating MRNA Methyl Cap Regulation, Function and Therapeutic Potential (Senior Non-Clinical Fellowship)
Cowling, V. (Investigator)
Medical Research Council
1/01/14 → 31/12/21
Project: Research

Cite this

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title = "c-Myc co-ordinates mRNA cap methylation and ribosomal RNA production",

abstract = "The mRNA cap is a structure added to RNA pol II transcripts in eukaryotes, which recruits factors involved in RNA processing, nuclear export and translation initiation. RNMT-RAM, the mRNA cap methyltransferase complex, completes the basic functional mRNA cap structure, cap 0, by methylating the cap guanosine. Here we report that RNMT-RAM co-ordinates mRNA processing with ribosome production. Suppression of RNMT-RAM reduces synthesis of the 45S ribosomal RNA (rRNA) precursor. RNMT- RAM is required for c-Myc expression, a major regulator of RNA pol I, which synthesises 45S rRNA. Constitutive expression of c-Myc restores rRNA synthesis when RNMT-RAM is suppressed, indicating that RNMT-RAM controls rRNA production predominantly by controlling c-Myc expression. We report that RNMT-RAM is recruited to the rDNA locus, which may contribute to rRNA synthesis in certain contexts.",

keywords = "RNMT-RAM, c-Myc, rRNA, transcription, mRNA cap, mRNA",

author = "Sianadh Dunn and Olivia Lombardi and Victoria Cowling",

note = "Research funded by Medical Research Council Senior Non-Clinical Fellowship MR/K024213/1 and Lister Prize Research Fellowship (VHC), MRC PhD studentship (SD), a Wellcome Trust Centre Award 097945/Z/11/Z and Wellcome Trust Strategic Award 100476/Z/12/Z and the Division of Signal Transduction Therapy, University of Dundee, funded by AstraZeneca, Boehringer-Ingelheim, GlaxoSmithKline, Janessen, Merck-Serono and Pfizer. ",

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doi = "10.1042/BCJ20160930",

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AU - Lombardi, Olivia

AU - Cowling, Victoria

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PY - 2017/1/20

Y1 - 2017/1/20

N2 - The mRNA cap is a structure added to RNA pol II transcripts in eukaryotes, which recruits factors involved in RNA processing, nuclear export and translation initiation. RNMT-RAM, the mRNA cap methyltransferase complex, completes the basic functional mRNA cap structure, cap 0, by methylating the cap guanosine. Here we report that RNMT-RAM co-ordinates mRNA processing with ribosome production. Suppression of RNMT-RAM reduces synthesis of the 45S ribosomal RNA (rRNA) precursor. RNMT- RAM is required for c-Myc expression, a major regulator of RNA pol I, which synthesises 45S rRNA. Constitutive expression of c-Myc restores rRNA synthesis when RNMT-RAM is suppressed, indicating that RNMT-RAM controls rRNA production predominantly by controlling c-Myc expression. We report that RNMT-RAM is recruited to the rDNA locus, which may contribute to rRNA synthesis in certain contexts.

AB - The mRNA cap is a structure added to RNA pol II transcripts in eukaryotes, which recruits factors involved in RNA processing, nuclear export and translation initiation. RNMT-RAM, the mRNA cap methyltransferase complex, completes the basic functional mRNA cap structure, cap 0, by methylating the cap guanosine. Here we report that RNMT-RAM co-ordinates mRNA processing with ribosome production. Suppression of RNMT-RAM reduces synthesis of the 45S ribosomal RNA (rRNA) precursor. RNMT- RAM is required for c-Myc expression, a major regulator of RNA pol I, which synthesises 45S rRNA. Constitutive expression of c-Myc restores rRNA synthesis when RNMT-RAM is suppressed, indicating that RNMT-RAM controls rRNA production predominantly by controlling c-Myc expression. We report that RNMT-RAM is recruited to the rDNA locus, which may contribute to rRNA synthesis in certain contexts.

KW - RNMT-RAM

KW - c-Myc

KW - rRNA

KW - transcription

KW - mRNA cap

KW - mRNA

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DO - 10.1042/BCJ20160930

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VL - 474

SP - 377

EP - 384

JO - Biochemical Journal

JF - Biochemical Journal

IS - 3

ER -

c-Myc co-ordinates mRNA cap methylation and ribosomal RNA production

Abstract

Keywords

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Projects

Investigating MRNA Methyl Cap Regulation, Function and Therapeutic Potential (Senior Non-Clinical Fellowship)

Cite this