c-Myc co-ordinates mRNA cap methylation and ribosomal RNA production

Sianadh Dunn, Olivia Lombardi, Victoria Cowling

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)
176 Downloads (Pure)

Abstract

The mRNA cap is a structure added to RNA pol II transcripts in eukaryotes, which recruits factors involved in RNA processing, nuclear export and translation initiation. RNMT-RAM, the mRNA cap methyltransferase complex, completes the basic functional mRNA cap structure, cap 0, by methylating the cap guanosine. Here we report that RNMT-RAM co-ordinates mRNA processing with ribosome production. Suppression of RNMT-RAM reduces synthesis of the 45S ribosomal RNA (rRNA) precursor. RNMT- RAM is required for c-Myc expression, a major regulator of RNA pol I, which synthesises 45S rRNA. Constitutive expression of c-Myc restores rRNA synthesis when RNMT-RAM is suppressed, indicating that RNMT-RAM controls rRNA production predominantly by controlling c-Myc expression. We report that RNMT-RAM is recruited to the rDNA locus, which may contribute to rRNA synthesis in certain contexts.
Original languageEnglish
Pages (from-to)377-384
Number of pages8
JournalBiochemical Journal
Volume474
Issue number3
Early online date1 Dec 2016
DOIs
Publication statusPublished - 20 Jan 2017

Keywords

  • RNMT-RAM
  • c-Myc
  • rRNA
  • transcription
  • mRNA cap
  • mRNA

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