Ca²⁺ buffer saturation underlies paired pulse facilitation in calbindin-D28k-containing terminals

Maria Blatow, Antonio Caputi, Nail Burnashev, Hannah Monyer, Andrei Rozov

    Research output: Contribution to journalArticlepeer-review

    189 Citations (Scopus)

    Abstract

    Ca²? buffer saturation was proposed as a mechanism of paired pulse facilitation (PPF). However, whether it operates under native conditions remained unclear. Here we show that saturation of the endogenous fast Ca²? buffer calbindin-D28k (CB) plays a major role in PPF at CB-containing synapses. Paired recordings from synaptically connected interneurons and pyramidal neurons in the mouse neocortex revealed that dialysis increased the amplitude of the first response and decreased PPF. Loading the presynaptic terminals with BAPTA or CB rescued the effect of the CB washout. We extended the study to the CB-positive facilitating excitatory mossy fiber-CA3 pyramidal cell synapse. The effects of different extracellular Ca²? concentrations and of EGTA indicated that PPF in CB-containing terminals depended on Ca²? influx rather than on the initial release probability. Experiments in CB knockout mice confirmed that buffer saturation is a novel basic presynaptic mechanism for activity-dependent control of synaptic gain.
    Original languageEnglish
    Pages (from-to)79-88
    Number of pages10
    JournalNeuron
    Volume38
    Issue number1
    DOIs
    Publication statusPublished - Apr 2003

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