Caenorhabditis elegans prom-1 is required for meiotic prophase progression and homologous chromosome pairing

Verena Jantsch, Lois Tang, Pawel Pasierbek, Alexandra Penkner, Sudhir Nayak, Antoine Baudrimont, Tim Schedl, Anton Gartner, Josef Loidl

    Research output: Contribution to journalArticle

    13 Citations (Scopus)

    Abstract

    A novel gene, prom-1, was isolated in a screen for Caenorhabditis elegans mutants with increased apoptosis in the germline. prom-1 encodes an F-box protein with limited homology to the putative human tumor suppressor FBXO47. Mutations in the prom-1 locus cause a strong reduction in bivalent formation, which results in increased embryonic lethality and a Him phenotype. Furthermore, retarded and asynchronous nuclear reorganization as well as reduced homologous synapsis occur during meiotic prophase. Accumulation of recombination protein RAD-51 in meiotic nuclei suggests disturbed repair of double-stranded DNA breaks. Nuclei in prom-1 mutant gonads timely complete mitotic proliferation and premeiotic replication, but they undergo prolonged delay upon meiotic entry. We, therefore, propose that prom-1 regulates the timely progression through meiotic prophase I and that in its absence the recognition of homologous chromosomes is strongly impaired.

    Original languageEnglish
    Pages (from-to)4911-4920
    Number of pages10
    JournalMolecular Biology of the Cell
    Volume18
    Issue number12
    DOIs
    Publication statusPublished - Dec 2007

    Keywords

    • CAENORHABDITIS-ELEGANS GERMLINE
    • DAMAGE-INDUCED APOPTOSIS
    • C-ELEGANS
    • SYNAPTONEMAL COMPLEX
    • NUCLEAR REORGANIZATION
    • CHIASMA FORMATION
    • CROSSING-OVER
    • GENE FAMILY
    • SYNAPSIS
    • RECOMBINATION

    Cite this

    Jantsch, V., Tang, L., Pasierbek, P., Penkner, A., Nayak, S., Baudrimont, A., ... Loidl, J. (2007). Caenorhabditis elegans prom-1 is required for meiotic prophase progression and homologous chromosome pairing. Molecular Biology of the Cell, 18(12), 4911-4920. https://doi.org/10.1091/mbc.E07-03-0243
    Jantsch, Verena ; Tang, Lois ; Pasierbek, Pawel ; Penkner, Alexandra ; Nayak, Sudhir ; Baudrimont, Antoine ; Schedl, Tim ; Gartner, Anton ; Loidl, Josef. / Caenorhabditis elegans prom-1 is required for meiotic prophase progression and homologous chromosome pairing. In: Molecular Biology of the Cell. 2007 ; Vol. 18, No. 12. pp. 4911-4920.
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    abstract = "A novel gene, prom-1, was isolated in a screen for Caenorhabditis elegans mutants with increased apoptosis in the germline. prom-1 encodes an F-box protein with limited homology to the putative human tumor suppressor FBXO47. Mutations in the prom-1 locus cause a strong reduction in bivalent formation, which results in increased embryonic lethality and a Him phenotype. Furthermore, retarded and asynchronous nuclear reorganization as well as reduced homologous synapsis occur during meiotic prophase. Accumulation of recombination protein RAD-51 in meiotic nuclei suggests disturbed repair of double-stranded DNA breaks. Nuclei in prom-1 mutant gonads timely complete mitotic proliferation and premeiotic replication, but they undergo prolonged delay upon meiotic entry. We, therefore, propose that prom-1 regulates the timely progression through meiotic prophase I and that in its absence the recognition of homologous chromosomes is strongly impaired.",
    keywords = "CAENORHABDITIS-ELEGANS GERMLINE, DAMAGE-INDUCED APOPTOSIS, C-ELEGANS, SYNAPTONEMAL COMPLEX, NUCLEAR REORGANIZATION, CHIASMA FORMATION, CROSSING-OVER, GENE FAMILY, SYNAPSIS, RECOMBINATION",
    author = "Verena Jantsch and Lois Tang and Pawel Pasierbek and Alexandra Penkner and Sudhir Nayak and Antoine Baudrimont and Tim Schedl and Anton Gartner and Josef Loidl",
    year = "2007",
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    Jantsch, V, Tang, L, Pasierbek, P, Penkner, A, Nayak, S, Baudrimont, A, Schedl, T, Gartner, A & Loidl, J 2007, 'Caenorhabditis elegans prom-1 is required for meiotic prophase progression and homologous chromosome pairing', Molecular Biology of the Cell, vol. 18, no. 12, pp. 4911-4920. https://doi.org/10.1091/mbc.E07-03-0243

    Caenorhabditis elegans prom-1 is required for meiotic prophase progression and homologous chromosome pairing. / Jantsch, Verena; Tang, Lois; Pasierbek, Pawel; Penkner, Alexandra; Nayak, Sudhir; Baudrimont, Antoine; Schedl, Tim; Gartner, Anton; Loidl, Josef.

    In: Molecular Biology of the Cell, Vol. 18, No. 12, 12.2007, p. 4911-4920.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Caenorhabditis elegans prom-1 is required for meiotic prophase progression and homologous chromosome pairing

    AU - Jantsch, Verena

    AU - Tang, Lois

    AU - Pasierbek, Pawel

    AU - Penkner, Alexandra

    AU - Nayak, Sudhir

    AU - Baudrimont, Antoine

    AU - Schedl, Tim

    AU - Gartner, Anton

    AU - Loidl, Josef

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    N2 - A novel gene, prom-1, was isolated in a screen for Caenorhabditis elegans mutants with increased apoptosis in the germline. prom-1 encodes an F-box protein with limited homology to the putative human tumor suppressor FBXO47. Mutations in the prom-1 locus cause a strong reduction in bivalent formation, which results in increased embryonic lethality and a Him phenotype. Furthermore, retarded and asynchronous nuclear reorganization as well as reduced homologous synapsis occur during meiotic prophase. Accumulation of recombination protein RAD-51 in meiotic nuclei suggests disturbed repair of double-stranded DNA breaks. Nuclei in prom-1 mutant gonads timely complete mitotic proliferation and premeiotic replication, but they undergo prolonged delay upon meiotic entry. We, therefore, propose that prom-1 regulates the timely progression through meiotic prophase I and that in its absence the recognition of homologous chromosomes is strongly impaired.

    AB - A novel gene, prom-1, was isolated in a screen for Caenorhabditis elegans mutants with increased apoptosis in the germline. prom-1 encodes an F-box protein with limited homology to the putative human tumor suppressor FBXO47. Mutations in the prom-1 locus cause a strong reduction in bivalent formation, which results in increased embryonic lethality and a Him phenotype. Furthermore, retarded and asynchronous nuclear reorganization as well as reduced homologous synapsis occur during meiotic prophase. Accumulation of recombination protein RAD-51 in meiotic nuclei suggests disturbed repair of double-stranded DNA breaks. Nuclei in prom-1 mutant gonads timely complete mitotic proliferation and premeiotic replication, but they undergo prolonged delay upon meiotic entry. We, therefore, propose that prom-1 regulates the timely progression through meiotic prophase I and that in its absence the recognition of homologous chromosomes is strongly impaired.

    KW - CAENORHABDITIS-ELEGANS GERMLINE

    KW - DAMAGE-INDUCED APOPTOSIS

    KW - C-ELEGANS

    KW - SYNAPTONEMAL COMPLEX

    KW - NUCLEAR REORGANIZATION

    KW - CHIASMA FORMATION

    KW - CROSSING-OVER

    KW - GENE FAMILY

    KW - SYNAPSIS

    KW - RECOMBINATION

    U2 - 10.1091/mbc.E07-03-0243

    DO - 10.1091/mbc.E07-03-0243

    M3 - Article

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    SP - 4911

    EP - 4920

    JO - Molecular Biology of the Cell

    JF - Molecular Biology of the Cell

    SN - 1059-1524

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    ER -