Caenorhabditis elegans prom-1 is required for meiotic prophase progression and homologous chromosome pairing

Verena Jantsch, Lois Tang, Pawel Pasierbek, Alexandra Penkner, Sudhir Nayak, Antoine Baudrimont, Tim Schedl, Anton Gartner, Josef Loidl

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    16 Citations (Scopus)

    Abstract

    A novel gene, prom-1, was isolated in a screen for Caenorhabditis elegans mutants with increased apoptosis in the germline. prom-1 encodes an F-box protein with limited homology to the putative human tumor suppressor FBXO47. Mutations in the prom-1 locus cause a strong reduction in bivalent formation, which results in increased embryonic lethality and a Him phenotype. Furthermore, retarded and asynchronous nuclear reorganization as well as reduced homologous synapsis occur during meiotic prophase. Accumulation of recombination protein RAD-51 in meiotic nuclei suggests disturbed repair of double-stranded DNA breaks. Nuclei in prom-1 mutant gonads timely complete mitotic proliferation and premeiotic replication, but they undergo prolonged delay upon meiotic entry. We, therefore, propose that prom-1 regulates the timely progression through meiotic prophase I and that in its absence the recognition of homologous chromosomes is strongly impaired.

    Original languageEnglish
    Pages (from-to)4911-4920
    Number of pages10
    JournalMolecular Biology of the Cell
    Volume18
    Issue number12
    DOIs
    Publication statusPublished - Dec 2007

    Keywords

    • CAENORHABDITIS-ELEGANS GERMLINE
    • DAMAGE-INDUCED APOPTOSIS
    • C-ELEGANS
    • SYNAPTONEMAL COMPLEX
    • NUCLEAR REORGANIZATION
    • CHIASMA FORMATION
    • CROSSING-OVER
    • GENE FAMILY
    • SYNAPSIS
    • RECOMBINATION

    Cite this

    Jantsch, V., Tang, L., Pasierbek, P., Penkner, A., Nayak, S., Baudrimont, A., Schedl, T., Gartner, A., & Loidl, J. (2007). Caenorhabditis elegans prom-1 is required for meiotic prophase progression and homologous chromosome pairing. Molecular Biology of the Cell, 18(12), 4911-4920. https://doi.org/10.1091/mbc.E07-03-0243