Caffeoyl shikimate esterase (CSE) is an enzyme in the lignin biosynthetic pathway in Arabidopsis

Ruben Vanholme; Igor Cesarino; Katarzyna Rataj; Yuguo Xiao; Lisa Sundin; Geert Goeminne; Hoon Kim; Joanna Cross; Kris Morreel; Pedro Araujo; Lydia Welsh; Jurgen Haustraete; Christopher McClellan; Bartel Vanholme; John Ralph; Gordon G. Simpson; Claire Halpin; Wout Boerjan

doi:10.1126/science.1241602

Caffeoyl shikimate esterase (CSE) is an enzyme in the lignin biosynthetic pathway in Arabidopsis

Ruben Vanholme, Igor Cesarino, Katarzyna Rataj, Yuguo Xiao, Lisa Sundin, Geert Goeminne, Hoon Kim, Joanna Cross, Kris Morreel, Pedro Araujo, Lydia Welsh, Jurgen Haustraete, Christopher McClellan, Bartel Vanholme, John Ralph, Gordon G. Simpson, Claire Halpin (Lead / Corresponding author), Wout Boerjan (Lead / Corresponding author)

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420 Citations (Scopus)

Abstract

Lignin is a major component of plant secondary cell walls. Here we describe caffeoyl shikimate esterase (CSE) as an enzyme central to the lignin biosynthetic pathway. Arabidopsis thaliana cse mutants deposit less lignin than do wild-type plants, and the remaining lignin is enriched in p-hydroxyphenyl units. Phenolic metabolite profiling identified accumulation of the lignin pathway intermediate caffeoyl shikimate in cse mutants as compared to caffeoyl shikimate levels in the wild type, suggesting caffeoyl shikimate as a substrate for CSE. Accordingly, recombinant CSE hydrolyzed caffeoyl shikimate into caffeate. Associated with the changes in lignin, the conversion of cellulose to glucose in cse mutants increased up to fourfold as compared to that in the wild type upon saccharification without pretreatment. Collectively, these data necessitate the revision of currently accepted models of the lignin biosynthetic pathway.

Original language	English
Pages (from-to)	1103-1106
Number of pages	4
Journal	Science
Volume	341
Issue number	6150
DOIs	https://doi.org/10.1126/science.1241602
Publication status	Published - 6 Sept 2013

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http://www.sciencemag.org/content/341/6150.toc

Aref#d: 20552. Manipulating Lignin to Improve Biofuel Conversion of Plant Biomass (joint with University of York)
Halpin, C. (Investigator)
20/04/09 → 19/10/14
Project: Research

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Vanholme, R., Cesarino, I., Rataj, K., Xiao, Y., Sundin, L., Goeminne, G., Kim, H., Cross, J., Morreel, K., Araujo, P., Welsh, L., Haustraete, J., McClellan, C., Vanholme, B., Ralph, J., Simpson, G. G., Halpin, C., & Boerjan, W. (2013). Caffeoyl shikimate esterase (CSE) is an enzyme in the lignin biosynthetic pathway in Arabidopsis. Science, 341(6150), 1103-1106. https://doi.org/10.1126/science.1241602

@article{3cda81c9b7e2444b8b8078aaf5f19d55,

title = "Caffeoyl shikimate esterase (CSE) is an enzyme in the lignin biosynthetic pathway in Arabidopsis",

abstract = "Lignin is a major component of plant secondary cell walls. Here we describe caffeoyl shikimate esterase (CSE) as an enzyme central to the lignin biosynthetic pathway. Arabidopsis thaliana cse mutants deposit less lignin than do wild-type plants, and the remaining lignin is enriched in p-hydroxyphenyl units. Phenolic metabolite profiling identified accumulation of the lignin pathway intermediate caffeoyl shikimate in cse mutants as compared to caffeoyl shikimate levels in the wild type, suggesting caffeoyl shikimate as a substrate for CSE. Accordingly, recombinant CSE hydrolyzed caffeoyl shikimate into caffeate. Associated with the changes in lignin, the conversion of cellulose to glucose in cse mutants increased up to fourfold as compared to that in the wild type upon saccharification without pretreatment. Collectively, these data necessitate the revision of currently accepted models of the lignin biosynthetic pathway.",

author = "Ruben Vanholme and Igor Cesarino and Katarzyna Rataj and Yuguo Xiao and Lisa Sundin and Geert Goeminne and Hoon Kim and Joanna Cross and Kris Morreel and Pedro Araujo and Lydia Welsh and Jurgen Haustraete and Christopher McClellan and Bartel Vanholme and John Ralph and Simpson, {Gordon G.} and Claire Halpin and Wout Boerjan",

year = "2013",

month = sep,

day = "6",

doi = "10.1126/science.1241602",

language = "English",

volume = "341",

pages = "1103--1106",

journal = "Science",

issn = "0036-8075",

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Vanholme, R, Cesarino, I, Rataj, K, Xiao, Y, Sundin, L, Goeminne, G, Kim, H, Cross, J, Morreel, K, Araujo, P, Welsh, L, Haustraete, J, McClellan, C, Vanholme, B, Ralph, J, Simpson, GG , Halpin, C & Boerjan, W 2013, 'Caffeoyl shikimate esterase (CSE) is an enzyme in the lignin biosynthetic pathway in Arabidopsis', Science, vol. 341, no. 6150, pp. 1103-1106. https://doi.org/10.1126/science.1241602

TY - JOUR

T1 - Caffeoyl shikimate esterase (CSE) is an enzyme in the lignin biosynthetic pathway in Arabidopsis

AU - Vanholme, Ruben

AU - Cesarino, Igor

AU - Rataj, Katarzyna

AU - Xiao, Yuguo

AU - Sundin, Lisa

AU - Goeminne, Geert

AU - Kim, Hoon

AU - Cross, Joanna

AU - Morreel, Kris

AU - Araujo, Pedro

AU - Welsh, Lydia

AU - Haustraete, Jurgen

AU - McClellan, Christopher

AU - Vanholme, Bartel

AU - Ralph, John

AU - Simpson, Gordon G.

AU - Halpin, Claire

AU - Boerjan, Wout

PY - 2013/9/6

Y1 - 2013/9/6

N2 - Lignin is a major component of plant secondary cell walls. Here we describe caffeoyl shikimate esterase (CSE) as an enzyme central to the lignin biosynthetic pathway. Arabidopsis thaliana cse mutants deposit less lignin than do wild-type plants, and the remaining lignin is enriched in p-hydroxyphenyl units. Phenolic metabolite profiling identified accumulation of the lignin pathway intermediate caffeoyl shikimate in cse mutants as compared to caffeoyl shikimate levels in the wild type, suggesting caffeoyl shikimate as a substrate for CSE. Accordingly, recombinant CSE hydrolyzed caffeoyl shikimate into caffeate. Associated with the changes in lignin, the conversion of cellulose to glucose in cse mutants increased up to fourfold as compared to that in the wild type upon saccharification without pretreatment. Collectively, these data necessitate the revision of currently accepted models of the lignin biosynthetic pathway.

AB - Lignin is a major component of plant secondary cell walls. Here we describe caffeoyl shikimate esterase (CSE) as an enzyme central to the lignin biosynthetic pathway. Arabidopsis thaliana cse mutants deposit less lignin than do wild-type plants, and the remaining lignin is enriched in p-hydroxyphenyl units. Phenolic metabolite profiling identified accumulation of the lignin pathway intermediate caffeoyl shikimate in cse mutants as compared to caffeoyl shikimate levels in the wild type, suggesting caffeoyl shikimate as a substrate for CSE. Accordingly, recombinant CSE hydrolyzed caffeoyl shikimate into caffeate. Associated with the changes in lignin, the conversion of cellulose to glucose in cse mutants increased up to fourfold as compared to that in the wild type upon saccharification without pretreatment. Collectively, these data necessitate the revision of currently accepted models of the lignin biosynthetic pathway.

U2 - 10.1126/science.1241602

DO - 10.1126/science.1241602

M3 - Article

C2 - 23950498

SN - 0036-8075

VL - 341

SP - 1103

EP - 1106

JO - Science

JF - Science

IS - 6150

ER -

Caffeoyl shikimate esterase (CSE) is an enzyme in the lignin biosynthetic pathway in Arabidopsis

Abstract

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Aref#d: 20552. Manipulating Lignin to Improve Biofuel Conversion of Plant Biomass (joint with University of York)

Cite this