Caffeoyl shikimate esterase (CSE) is an enzyme in the lignin biosynthetic pathway in Arabidopsis

Ruben Vanholme, Igor Cesarino, Katarzyna Rataj, Yuguo Xiao, Lisa Sundin, Geert Goeminne, Hoon Kim, Joanna Cross, Kris Morreel, Pedro Araujo, Lydia Welsh, Jurgen Haustraete, Christopher McClellan, Bartel Vanholme, John Ralph, Gordon G. Simpson, Claire Halpin (Lead / Corresponding author), Wout Boerjan (Lead / Corresponding author)

    Research output: Contribution to journalArticle

    171 Citations (Scopus)

    Abstract

    Lignin is a major component of plant secondary cell walls. Here we describe caffeoyl shikimate esterase (CSE) as an enzyme central to the lignin biosynthetic pathway. Arabidopsis thaliana cse mutants deposit less lignin than do wild-type plants, and the remaining lignin is enriched in p-hydroxyphenyl units. Phenolic metabolite profiling identified accumulation of the lignin pathway intermediate caffeoyl shikimate in cse mutants as compared to caffeoyl shikimate levels in the wild type, suggesting caffeoyl shikimate as a substrate for CSE. Accordingly, recombinant CSE hydrolyzed caffeoyl shikimate into caffeate. Associated with the changes in lignin, the conversion of cellulose to glucose in cse mutants increased up to fourfold as compared to that in the wild type upon saccharification without pretreatment. Collectively, these data necessitate the revision of currently accepted models of the lignin biosynthetic pathway.
    Original languageEnglish
    Pages (from-to)1103-1106
    Number of pages4
    JournalScience
    Volume341
    Issue number6150
    DOIs
    Publication statusPublished - 6 Sep 2013

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    Lignin
    Biosynthetic Pathways
    Esterases
    Arabidopsis
    Enzymes
    Plant Cells
    Cellulose
    Cell Wall
    Glucose

    Cite this

    Vanholme, R., Cesarino, I., Rataj, K., Xiao, Y., Sundin, L., Goeminne, G., ... Boerjan, W. (2013). Caffeoyl shikimate esterase (CSE) is an enzyme in the lignin biosynthetic pathway in Arabidopsis. Science, 341(6150), 1103-1106. https://doi.org/10.1126/science.1241602
    Vanholme, Ruben ; Cesarino, Igor ; Rataj, Katarzyna ; Xiao, Yuguo ; Sundin, Lisa ; Goeminne, Geert ; Kim, Hoon ; Cross, Joanna ; Morreel, Kris ; Araujo, Pedro ; Welsh, Lydia ; Haustraete, Jurgen ; McClellan, Christopher ; Vanholme, Bartel ; Ralph, John ; Simpson, Gordon G. ; Halpin, Claire ; Boerjan, Wout. / Caffeoyl shikimate esterase (CSE) is an enzyme in the lignin biosynthetic pathway in Arabidopsis. In: Science. 2013 ; Vol. 341, No. 6150. pp. 1103-1106.
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    title = "Caffeoyl shikimate esterase (CSE) is an enzyme in the lignin biosynthetic pathway in Arabidopsis",
    abstract = "Lignin is a major component of plant secondary cell walls. Here we describe caffeoyl shikimate esterase (CSE) as an enzyme central to the lignin biosynthetic pathway. Arabidopsis thaliana cse mutants deposit less lignin than do wild-type plants, and the remaining lignin is enriched in p-hydroxyphenyl units. Phenolic metabolite profiling identified accumulation of the lignin pathway intermediate caffeoyl shikimate in cse mutants as compared to caffeoyl shikimate levels in the wild type, suggesting caffeoyl shikimate as a substrate for CSE. Accordingly, recombinant CSE hydrolyzed caffeoyl shikimate into caffeate. Associated with the changes in lignin, the conversion of cellulose to glucose in cse mutants increased up to fourfold as compared to that in the wild type upon saccharification without pretreatment. Collectively, these data necessitate the revision of currently accepted models of the lignin biosynthetic pathway.",
    author = "Ruben Vanholme and Igor Cesarino and Katarzyna Rataj and Yuguo Xiao and Lisa Sundin and Geert Goeminne and Hoon Kim and Joanna Cross and Kris Morreel and Pedro Araujo and Lydia Welsh and Jurgen Haustraete and Christopher McClellan and Bartel Vanholme and John Ralph and Simpson, {Gordon G.} and Claire Halpin and Wout Boerjan",
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    Vanholme, R, Cesarino, I, Rataj, K, Xiao, Y, Sundin, L, Goeminne, G, Kim, H, Cross, J, Morreel, K, Araujo, P, Welsh, L, Haustraete, J, McClellan, C, Vanholme, B, Ralph, J, Simpson, GG, Halpin, C & Boerjan, W 2013, 'Caffeoyl shikimate esterase (CSE) is an enzyme in the lignin biosynthetic pathway in Arabidopsis', Science, vol. 341, no. 6150, pp. 1103-1106. https://doi.org/10.1126/science.1241602

    Caffeoyl shikimate esterase (CSE) is an enzyme in the lignin biosynthetic pathway in Arabidopsis. / Vanholme, Ruben; Cesarino, Igor; Rataj, Katarzyna; Xiao, Yuguo; Sundin, Lisa; Goeminne, Geert; Kim, Hoon; Cross, Joanna; Morreel, Kris; Araujo, Pedro; Welsh, Lydia; Haustraete, Jurgen; McClellan, Christopher; Vanholme, Bartel; Ralph, John; Simpson, Gordon G.; Halpin, Claire (Lead / Corresponding author); Boerjan, Wout (Lead / Corresponding author).

    In: Science, Vol. 341, No. 6150, 06.09.2013, p. 1103-1106.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Caffeoyl shikimate esterase (CSE) is an enzyme in the lignin biosynthetic pathway in Arabidopsis

    AU - Vanholme, Ruben

    AU - Cesarino, Igor

    AU - Rataj, Katarzyna

    AU - Xiao, Yuguo

    AU - Sundin, Lisa

    AU - Goeminne, Geert

    AU - Kim, Hoon

    AU - Cross, Joanna

    AU - Morreel, Kris

    AU - Araujo, Pedro

    AU - Welsh, Lydia

    AU - Haustraete, Jurgen

    AU - McClellan, Christopher

    AU - Vanholme, Bartel

    AU - Ralph, John

    AU - Simpson, Gordon G.

    AU - Halpin, Claire

    AU - Boerjan, Wout

    PY - 2013/9/6

    Y1 - 2013/9/6

    N2 - Lignin is a major component of plant secondary cell walls. Here we describe caffeoyl shikimate esterase (CSE) as an enzyme central to the lignin biosynthetic pathway. Arabidopsis thaliana cse mutants deposit less lignin than do wild-type plants, and the remaining lignin is enriched in p-hydroxyphenyl units. Phenolic metabolite profiling identified accumulation of the lignin pathway intermediate caffeoyl shikimate in cse mutants as compared to caffeoyl shikimate levels in the wild type, suggesting caffeoyl shikimate as a substrate for CSE. Accordingly, recombinant CSE hydrolyzed caffeoyl shikimate into caffeate. Associated with the changes in lignin, the conversion of cellulose to glucose in cse mutants increased up to fourfold as compared to that in the wild type upon saccharification without pretreatment. Collectively, these data necessitate the revision of currently accepted models of the lignin biosynthetic pathway.

    AB - Lignin is a major component of plant secondary cell walls. Here we describe caffeoyl shikimate esterase (CSE) as an enzyme central to the lignin biosynthetic pathway. Arabidopsis thaliana cse mutants deposit less lignin than do wild-type plants, and the remaining lignin is enriched in p-hydroxyphenyl units. Phenolic metabolite profiling identified accumulation of the lignin pathway intermediate caffeoyl shikimate in cse mutants as compared to caffeoyl shikimate levels in the wild type, suggesting caffeoyl shikimate as a substrate for CSE. Accordingly, recombinant CSE hydrolyzed caffeoyl shikimate into caffeate. Associated with the changes in lignin, the conversion of cellulose to glucose in cse mutants increased up to fourfold as compared to that in the wild type upon saccharification without pretreatment. Collectively, these data necessitate the revision of currently accepted models of the lignin biosynthetic pathway.

    U2 - 10.1126/science.1241602

    DO - 10.1126/science.1241602

    M3 - Article

    VL - 341

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    EP - 1106

    JO - Science

    JF - Science

    SN - 0036-8075

    IS - 6150

    ER -

    Vanholme R, Cesarino I, Rataj K, Xiao Y, Sundin L, Goeminne G et al. Caffeoyl shikimate esterase (CSE) is an enzyme in the lignin biosynthetic pathway in Arabidopsis. Science. 2013 Sep 6;341(6150):1103-1106. https://doi.org/10.1126/science.1241602