Cajal bodies and the nucleolus are required for a plant virus systemic infection

Sang Hyon Kim, Eugene V. Ryabov, Natalia O. Kalinina, Daria V. Rakitina, Trudi Gillespie, Stuart Macfarlane, Sophie Haupt, John W. S. Brown, Michael Taliansky

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    Abstract

    The nucleolus and Cajal bodies (CBs) are prominent interacting subnuclear domains involved in a number of crucial aspects of cell function. Certain viruses interact with these compartments but the functions of such interactions are largely uncharacterized. Here, we show that the ability of the groundnut rosette virus open reading frame (ORF) 3 protein to move viral RNA long distances through the phloem strictly depends on its interaction with CBs and the nucleolus. The ORF3 protein targets and reorganizes CBs into multiple CB-like structures and then enters the nucleolus by causing fusion of these structures with the nucleolus. The nucleolar localization of the ORF3 protein is essential for subsequent formation of viral ribonucleoprotein (RNP) particles capable of virus long-distance movement and systemic infection. We provide a model whereby the ORF3 protein utilizes trafficking pathways involving CBs to enter the nucleolus and, along with fibrillarin, exit the nucleus to form viral ‘transportcompetent’ RNP particles in the cytoplasm.
    Original languageEnglish
    Pages (from-to)2169-2179
    Number of pages11
    JournalThe EMBO Journal
    Volume26
    Issue number8
    DOIs
    Publication statusPublished - Apr 2007

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    Keywords

    • Cajal bodies
    • Nucleolus
    • Ribonucleoprotein particles
    • Umbravirus

    Cite this

    Kim, S. H., Ryabov, E. V., Kalinina, N. O., Rakitina, D. V., Gillespie, T., Macfarlane, S., Haupt, S., Brown, J. W. S., & Taliansky, M. (2007). Cajal bodies and the nucleolus are required for a plant virus systemic infection. The EMBO Journal, 26(8), 2169-2179. https://doi.org/10.1038/sj.emboj.7601674